Cell Biology Antibodies 12
Anti-CLTB Antibody (CAB8404)
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- SKU:
- CAB8404
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IF
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-CLTB Antibody |
| Antibody SKU: | CAB8404 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human CLTB (NP_001825.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | LO2, A-549, MCF7, SW480, NIH/3T3, Mouse kidney, Mouse lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human CLTB (NP_001825.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MADD FGFF SSSE SGAP EAAE EDPA AAFL AQQE SEIA GIEN DEGF GAPA GSHA APAQ PGPT SGAG SEDM GTTV NGDV FQEA NGPA DGYA AIAQ ADRL TQEP ESIR KWRE EQRK RLQE LDAA SKVT EQEW REKA KKDL EEWN QRQS EQVE KNKI NNRA SEEA FVKE SKEE TPGT EWEK VAQL CDFN PKSS KQCK DVSR LRSV LMSL KQTP LSR |
| Gene ID: | 1212 |
| Uniprot: | P09497 |
| Cellular Location: | Cytoplasmic side, Cytoplasmic vesicle membrane, Membrane, Peripheral membrane protein, coated pit |
| Calculated MW: | 23kDa/25kDa |
| Observed MW: | 30kDa |
| Synonyms: | CLTB, LCB |
| Background: | Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | CLTB: Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Clathrin coats are formed from molecules containing 3 heavy chains and 3 light chains. Interacts (via N-terminus) with HIP1. Interacts with HIP1R. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Vesicle Chromosomal Location of Human Ortholog: 5q35 Cellular Component: clathrin coat; clathrin coat of coated pit; clathrin coat of trans-Golgi network vesicle; clathrin vesicle coat; cytoplasm; intracellular membrane-bound organelle; plasma membrane; trans-Golgi network Molecular Function:clathrin heavy chain binding; peptide binding; protein binding; structural molecule activity Biological Process: intracellular protein transport |
| NCBI Summary: | Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P09497 |
| NCBI GenInfo Identifier: | 4502901 |
| NCBI Gene ID: | 1212 |
| NCBI Accession: | NP_001825.1 |
| UniProt Secondary Accession: | P09497,Q53Y37, Q6FHW1, |
| UniProt Related Accession: | P09497 |
| Molecular Weight: | 23,181 Da |
| NCBI Full Name: | clathrin light chain B isoform a |
| NCBI Synonym Full Names: | clathrin light chain B |
| NCBI Official Symbol: | CLTB |
| NCBI Official Synonym Symbols: | LCB |
| NCBI Protein Information: | clathrin light chain B |
| UniProt Protein Name: | Clathrin light chain B |
| Protein Family: | Clathrin |
| UniProt Gene Name: | CLTB |
| UniProt Entry Name: | CLCB_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CLTB antibody (CAB8404) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of HeLa cells using Anti-CLTB Polyclonal Antibody (CAB8404) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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