KO Validated Antibodies 2

Anti-CLPP Antibody (CAB19975)[KO Validated]

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SKU:
CAB19975
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CLPP Antibody [KO Validated]
Antibody SKU:CAB19975
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human CLPP.
Application:WB
Recommended Dilution:WB 1:1000 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:293T
Immunogen:Recombinant protein of human CLPP.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:8192
Uniprot:Q16740
Cellular Location:Mitochondrion matrix
Calculated MW:30kDa
Observed MW:25KDa
Anti-CLPP Antibody [KO Validated] (CAB19975)Western blot analysis of extracts from normal (control) and CLPP knockout (KO) 293T cells, using CLPP antibody (CAB19975) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:CLPP, DFNB81, PRLTS3
Background:The protein encoded by this gene belongs to the peptidase family S14 and hydrolyzes proteins into small peptides in the presence of ATP and magnesium. The protein is transported into mitochondrial matrix and is associated with the inner mitochondrial membrane.
UniProt Protein Function:Protease component of the Clp complex that cleaves peptides and various proteins in an ATP-dependent process. Has low peptidase activity in the absence of CLPX. The Clp complex can degrade CSN1S1, CSN2 and CSN3, as well as synthetic peptides (in vitro) and may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates.
NCBI Summary:The protein encoded by this gene belongs to the peptidase family S14 and hydrolyzes proteins into small peptides in the presence of ATP and magnesium. The protein is transported into mitochondrial matrix and is associated with the inner mitochondrial membrane. [provided by RefSeq, Jul 2008]
UniProt Code:Q16740
NCBI GenInfo Identifier:5174419
NCBI Gene ID:8192
NCBI Accession:NP_006003
UniProt Secondary Accession:Q16740,B2R4W5,
UniProt Related Accession:Q16740
Molecular Weight:24.2kDa (222aa), confirmed by MALDI-TOF
NCBI Full Name:ATP-dependent Clp protease proteolytic subunit, mitochondrial
NCBI Synonym Full Names:caseinolytic mitochondrial matrix peptidase proteolytic subunit
NCBI Official Symbol:CLPP  
NCBI Official Synonym Symbols:DFNB81; PRLTS3  
NCBI Protein Information:ATP-dependent Clp protease proteolytic subunit, mitochondrial
UniProt Protein Name:ATP-dependent Clp protease proteolytic subunit, mitochondrial
UniProt Synonym Protein Names:Endopeptidase Clp
Protein Family:ATP-dependent Clp protease proteolytic
UniProt Gene Name:CLPP  
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