Antibody Name:	Anti-CLIC4 Antibody
Antibody SKU:	CAB7088
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-253 of human CLIC4 (NP_039234.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW480, HeLa, Jurkat, Raji, Mouse kidney, Mouse lung, Mouse small intestine, Mouse esophagus, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-253 of human CLIC4 (NP_039234.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MALS MPLN GLKE EDKE PLIE LFVK AGSD GESI GNCP FSQR LFMI LWLK GVVF SVTT VDLK RKPA DLQN LAPG THPP FITF NSEV KTDV NKIE EFLE EVLC PPKY LKLS PKHP ESNT AGMD IFAK FSAY IKNS RPEA NEAL ERGL LKTL QKLD EYLN SPLP DEID ENSM EDIK FSTR KFLD GNEM TLAD CNLL PKLH IVKV VAKK YRNF DIPK EMTG IWRY LTNA YSRD EFTN TCPS DKEV EIAY SDVA KRLT K
Gene ID:	25932
Uniprot:	Q9Y696
Cellular Location:	Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle membrane, Mitochondrion, Nucleus matrix, Single-pass membrane protein, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:	28kDa
Observed MW:	29kDa

Synonyms:

CLIC4, CLIC4L, H1, MTCLIC, huH1, p64H1

Background:

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells).

UniProt Protein Function:	CLIC4: Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell- surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical- basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis). Belongs to the chloride channel CLIC family.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 1p36.11 Cellular Component: centrosome; cell surface; nuclear matrix; microvillus; cytoplasmic vesicle membrane; mitochondrion; intercellular junction; cytosol; actin cytoskeleton; microtubule cytoskeleton; apical part of cell; perinuclear region of cytoplasm; cytoplasm; plasma membrane; intracellular; midbody Molecular Function:chloride channel activity; protein binding; voltage-gated ion channel activity Biological Process: keratinocyte differentiation; vacuolar acidification; fertilization; branching morphogenesis of a tube; apoptosis; multicellular organism growth; establishment and/or maintenance of apical/basal cell polarity; chloride transport; angiogenesis; endothelial cell morphogenesis; cell differentiation; negative regulation of cell migration; regulation of cytoskeleton organization and biogenesis
NCBI Summary:	Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells). [provided by RefSeq, Jul 2008]
UniProt Code:	Q9Y696
NCBI GenInfo Identifier:	20141285
NCBI Gene ID:	25932
NCBI Accession:	Q9Y696.4
UniProt Secondary Accession:	Q9Y696,Q9UFW9, Q9UQJ6,
UniProt Related Accession:	Q9Y696
Molecular Weight:	253
NCBI Full Name:	Chloride intracellular channel protein 4
NCBI Synonym Full Names:	chloride intracellular channel 4
NCBI Official Symbol:	CLIC4
NCBI Official Synonym Symbols:	H1; huH1; p64H1; CLIC4L; MTCLIC
NCBI Protein Information:	chloride intracellular channel protein 4; chloride intracellular channel 4 like; intracellular chloride ion channel protein p64H1
UniProt Protein Name:	Chloride intracellular channel protein 4
UniProt Synonym Protein Names:	Intracellular chloride ion channel protein p64H1
Protein Family:	Chloride intracellular channel protein
UniProt Gene Name:	CLIC4
UniProt Entry Name:	CLIC4_HUMAN

	Western blot analysis of extracts of various cell lines, using CLIC4 antibody (CAB7088) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 2s.
	Immunohistochemistry of paraffin-embedded rat brain using CLIC4 antibody (CAB7088) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A-549 cells using CLIC4 antibody (CAB7088). Blue: DAPI for nuclear staining.