Antibody Name:	CKMT2 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8088
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 40-230 of human CKMT2 (NP_001816.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:20 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Mouse skeletal muscle, Mouse heart, Mouse diaphragm, Rat skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 40-230 of human CKMT2 (NP_001816.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EVRE QPRL FPPS ADYP DLRK HNNC MAEC LTPA IYAK LRNK VTPN GYTL DQCI QTGV DNPG HPFI KTVG MVAG DEES YEVF ADLF DPVI KLRH NGYD PRVM KHTT DLDA SKIT QGQF DEHY VLSS RVRT GRSI RGLS LPPA CTRA ERRE VENV AITA LEGL KGDL AGRY YKLS EMTE QDQQ RLID DHFL FDK
Gene ID:	1160
Uniprot:	P17540
Cellular Location:	Intermembrane side, Mitochondrion inner membrane, Peripheral membrane protein
Calculated MW:	47kDa
Observed MW:	40kDa

Synonyms:

CKMT2, SMTCK

Background:

Mitochondrial creatine kinase (MtCK) is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Sarcomeric mitochondrial creatine kinase has 80% homology with the coding exons of ubiquitous mitochondrial creatine kinase. This gene contains sequences homologous to several motifs that are shared among some nuclear genes encoding mitochondrial proteins and thus may be essential for the coordinated activation of these genes during mitochondrial biogenesis. Three transcript variants encoding the same protein have been found for this gene.

UniProt Protein Function:	CKMT2: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family.
UniProt Protein Details:	Protein type:Mitochondrial; Amino Acid Metabolism - arginine and proline; Kinase, other; EC 2.7.3.2 Chromosomal Location of Human Ortholog: 5q13.3 Cellular Component: mitochondrial inner membrane; mitochondrion Molecular Function:creatine kinase activity Biological Process: creatine metabolic process; muscle contraction
NCBI Summary:	Mitochondrial creatine kinase (MtCK) is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Sarcomeric mitochondrial creatine kinase has 80% homology with the coding exons of ubiquitous mitochondrial creatine kinase. This gene contains sequences homologous to several motifs that are shared among some nuclear genes encoding mitochondrial proteins and thus may be essential for the coordinated activation of these genes during mitochondrial biogenesis. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P17540
NCBI GenInfo Identifier:	116242603
NCBI Gene ID:	1160
NCBI Accession:	P17540.2
UniProt Secondary Accession:	P17540,Q6ICS8, Q8N1E1,
UniProt Related Accession:	P17540
Molecular Weight:	47,504 Da
NCBI Full Name:	Creatine kinase S-type, mitochondrial
NCBI Synonym Full Names:	creatine kinase, mitochondrial 2
NCBI Official Symbol:	CKMT2
NCBI Official Synonym Symbols:	SMTCK
NCBI Protein Information:	creatine kinase S-type, mitochondrial
UniProt Protein Name:	Creatine kinase S-type, mitochondrial
UniProt Synonym Protein Names:	Basic-type mitochondrial creatine kinase; Mib-CK; Sarcomeric mitochondrial creatine kinase; S-MtCK
Protein Family:	Creatine kinase
UniProt Gene Name:	CKMT2
UniProt Entry Name:	KCRS_HUMAN

	Western blot analysis of extracts of various cell lines, using CKMT2 antibody (CAB8088) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded rat heart using CKMT2 antibody (CAB8088) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophagus using CKMT2 antibody (CAB8088) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using CKMT2 antibody (CAB8088) at dilution of 1:100 (40x lens).