Antibody Name:	Anti-CISH Antibody
Antibody SKU:	CAB14527
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 17-130 of human CISH (NP_659508.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 17-130 of human CISH (NP_659508.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TGQR PLWA PSLE LPKP VMQP LPAG AFLE EVAE GTPA QTES EPKV LDPE EDLL CIAK TFSY LRES GWYW GSIT ASEA RQHL QKMP EGTF LVRD STHP SYLF TLSV KTTR GPTN VR
Gene ID:	1154
Uniprot:	Q9NSE2
Cellular Location:
Calculated MW:	28kDa/29kDa/30kDa
Observed MW:	37kDa

Synonyms:

CISH, BACTS2, CIS, CIS-1, G18, SOCS

Background:

The protein encoded by this gene contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by IL2, IL3, GM-CSF and EPO in hematopoietic cells. Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	CISH: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate- recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Stably associated with the tyrosine-phosphorylated IL3 receptor beta chain and tyrosine-phosphorylated EPO receptor (EPOR). By a subset of cytokines including EPO/erythropoietin. Expressed in various epithelial tissues. Abundantly expressed in liver and kidney, and to a lesser extent in lung. The tissue distribution of isoforms 1 and 1B is distinct. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 3p21.3 Cellular Component: cytoplasm; plasma membrane; cytosol Molecular Function:protein binding; protein kinase inhibitor activity Biological Process: cytokine and chemokine mediated signaling pathway; protein kinase C activation; negative regulation of insulin receptor signaling pathway; negative regulation of protein kinase activity; protein ubiquitination; regulation of cell growth; negative regulation of JAK-STAT cascade Disease: Mycobacterium Tuberculosis, Susceptibility To; Bacteremia, Susceptibility To, 2; Malaria, Susceptibility To
NCBI Summary:	The protein encoded by this gene contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by IL2, IL3, GM-CSF and EPO in hematopoietic cells. Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
UniProt Code:	Q9NSE2
NCBI GenInfo Identifier:	13124022
NCBI Gene ID:	1154
NCBI Accession:	Q9NSE2.1
UniProt Secondary Accession:	Q9NSE2,Q9NS38, Q9Y5R1, B2R9N1, G5E9R1,
UniProt Related Accession:	Q9NSE2
Molecular Weight:	30,734 Da
NCBI Full Name:	Cytokine-inducible SH2-containing protein
NCBI Synonym Full Names:	cytokine inducible SH2-containing protein
NCBI Official Symbol:	CISH
NCBI Official Synonym Symbols:	CIS; G18; SOCS; CIS-1; BACTS2
NCBI Protein Information:	cytokine-inducible SH2-containing protein; suppressor of cytokine signaling; cytokine-inducible inhibitor of signaling type 1B
UniProt Protein Name:	Cytokine-inducible SH2-containing protein
UniProt Synonym Protein Names:	CIS-1; Protein G18; Suppressor of cytokine signaling; SOCS
Protein Family:	Cis-abienol synthase
UniProt Gene Name:	CISH
UniProt Entry Name:	CISH_HUMAN

	Western blot analysis of extracts of 293T cells, using CISH antibody (CAB14527) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunofluorescence analysis of C6 cells using CISH antibody (CAB14527) at dilution of 1:100. Blue: DAPI for nuclear staining.