Cell Biology Antibodies 12

Anti-CHST15 Antibody (CAB8984)

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SKU:
CAB8984
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CHST15 Antibody
Antibody SKU:CAB8984
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 452-561 of human CHST15 (NP_056976.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Rat
Positive Samples:Raji, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 452-561 of human CHST15 (NP_056976.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LQVG LYAV YLLD WLSV FDKQ QFLI LRLE DHAS NVKY TMHK VFQF LNLG PLSE KQEA LMTK SPAS NARR PEDR NLGP MWPI TQKI LRDF YRPF NARL AQVL ADEA FAWK TT
Gene ID:51363
Uniprot:Q7LFX5
Cellular Location:Golgi apparatus membrane, Single-pass type II membrane protein
Calculated MW:58kDa/64kDa
Observed MW:80kDa
Synonyms:CHST15, BRAG, GALNAC4S-6ST
Background:Chondroitin sulfate (CS) is a glycosaminoglycan which is an important structural component of the extracellular matrix and which links to proteins to form proteoglycans. Chondroitin sulfate E (CS-E) is an isomer of chondroitin sulfate in which the C-4 and C-6 hydroxyl groups are sulfated. This gene encodes a type II transmembrane glycoprotein that acts as a sulfotransferase to transfer sulfate to the C-6 hydroxal group of chondroitin sulfate. This gene has also been identified as being co-expressed with RAG1 in B-cells and as potentially acting as a B-cell surface signaling receptor. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
UniProt Protein Function:CHST15: Sulfotransferase that transfers sulfate from 3'- phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO(4)) repeating units. It also transfers sulfate to a unique non- reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. May also act as a B- cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo. Belongs to the sulfotransferase 1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 2.8.2.33; Transferase; Glycan Metabolism - chondroitin sulfate biosynthesis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q26

Cellular Component: Golgi membrane; integral to membrane

Molecular Function:N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase activity; 3'-phosphoadenosine 5'-phosphosulfate binding

Biological Process: chondroitin sulfate metabolic process; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; hexose biosynthetic process; pathogenesis

NCBI Summary:Chondroitin sulfate (CS) is a glycosaminoglycan which is an important structural component of the extracellular matrix and which links to proteins to form proteoglycans. Chondroitin sulfate E (CS-E) is an isomer of chondroitin sulfate in which the C-4 and C-6 hydroxyl groups are sulfated. This gene encodes a type II transmembrane glycoprotein that acts as a sulfotransferase to transfer sulfate to the C-6 hydroxal group of chondroitin sulfate. This gene has also been identified as being co-expressed with RAG1 in B-cells and as potentially acting as a B-cell surface signaling receptor. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012]
UniProt Code:Q7LFX5
NCBI GenInfo Identifier:74749920
NCBI Gene ID:51363
NCBI Accession:Q7LFX5.1
UniProt Secondary Accession:Q7LFX5,O60338, O60474, Q86VM4,
UniProt Related Accession:Q7LFX5
Molecular Weight:58,109 Da
NCBI Full Name:Carbohydrate sulfotransferase 15
NCBI Synonym Full Names:carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15
NCBI Official Symbol:CHST15  
NCBI Official Synonym Symbols:BRAG; GALNAC4S-6ST  
NCBI Protein Information:carbohydrate sulfotransferase 15; B cell RAG associated protein (GALNAC4S-6ST); B-cell RAG-associated gene protein; hBRAG
UniProt Protein Name:Carbohydrate sulfotransferase 15
UniProt Synonym Protein Names:B-cell RAG-associated gene protein; hBRAG; N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase; GalNAc4S-6ST
Protein Family:Carbohydrate sulfotransferase
UniProt Gene Name:CHST15  
UniProt Entry Name:CHSTF_HUMAN
Anti-CHST15 Polyclonal Antibody (CAB8984)Western blot analysis of extracts of various cell lines, using CHST15 antibody (CAB8984) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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