Antibody Name:	Anti-CHRNB2 Antibody
Antibody SKU:	CAB9808
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 340-430 of human CHRNB2 (NP_000739.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, SH-SY5Y, Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 340-430 of human CHRNB2 (NP_000739.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LEKL PALL FMQQ PRHH CARQ RLRL RRRQ RERE GAGA LFFR EAPG ADSC TCFV NRAS VQGL AGAF GAEP APVA GPGR SGEP CGCG LREA VDG
Gene ID:	1141
Uniprot:	P17787
Cellular Location:	Cell junction, Cell membrane, Multi-pass membrane protein, postsynaptic cell membrane, synapse
Calculated MW:	57kDa
Observed MW:	50-52kDa

Synonyms:

CHRNB2, EFNL3, nAChRB2

Background:

Neuronal acetylcholine receptors are homo- or heteropentameric complexes composed of homologous alpha and beta subunits. They belong to a superfamily of ligand-gated ion channels which allow the flow of sodium and potassium across the plasma membrane in response to ligands such as acetylcholine and nicotine. This gene encodes one of several beta subunits. Mutations in this gene are associated with autosomal dominant nocturnal frontal lobe epilepsy.

UniProt Protein Function:	nAChRB2: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Defects in CHRNB2 are the cause of nocturnal frontal lobe epilepsy type 3 (ENFL3). ENFL3 is an autosomal dominant epilepsy characterized by nocturnal seizures with hyperkinetic automatisms and poorly organized stereotyped movements. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta- 2/CHRNB2 sub-subfamily.
UniProt Protein Details:	Protein type:Receptor, misc.; Channel, cation; Membrane protein, multi-pass; Channel, ligand-gated; Membrane protein, integral Chromosomal Location of Human Ortholog: 1q21.3 Cellular Component: external side of plasma membrane; nicotinic acetylcholine-gated receptor-channel complex; plasma membrane Molecular Function:acetylcholine binding; acetylcholine receptor activity; ligand-gated ion channel activity; nicotinic acetylcholine-activated cation-selective channel activity; protein binding Biological Process: associative learning; B cell activation; behavioral response to nicotine; calcium ion transport; central nervous system projection neuron axonogenesis; cognition; lateral geniculate nucleus development; learning; locomotory behavior; membrane depolarization; memory; negative regulation of action potential; neurological system process; neuromuscular synaptic transmission; optic nerve morphogenesis; positive regulation of B cell proliferation; positive regulation of dopamine secretion; regulation of circadian sleep/wake cycle, REM sleep; regulation of dendrite morphogenesis; regulation of dopamine metabolic process; regulation of dopamine secretion; regulation of synaptic transmission, dopaminergic; regulation of synaptogenesis; response to cocaine; response to ethanol; response to hypoxia; response to nicotine; sensory perception of pain; sensory perception of sound; signal transduction; smooth muscle contraction; social behavior; synaptic transmission involved in micturition; synaptic transmission, cholinergic; vestibulocochlear nerve development; visual learning; visual perception Disease: Epilepsy, Nocturnal Frontal Lobe, 3
NCBI Summary:	Neuronal acetylcholine receptors are homo- or heteropentameric complexes composed of homologous alpha and beta subunits. They belong to a superfamily of ligand-gated ion channels which allow the flow of sodium and potassium across the plasma membrane in response to ligands such as acetylcholine and nicotine. This gene encodes one of several beta subunits. Mutations in this gene are associated with autosomal dominant nocturnal frontal lobe epilepsy. [provided by RefSeq, Jul 2008]
UniProt Code:	P17787
NCBI GenInfo Identifier:	113105
NCBI Gene ID:	1141
NCBI Accession:	P17787.1
UniProt Secondary Accession:	P17787,Q9UEH9,
UniProt Related Accession:	P17787
Molecular Weight:	57,019 Da
NCBI Full Name:	Neuronal acetylcholine receptor subunit beta-2
NCBI Synonym Full Names:	cholinergic receptor nicotinic beta 2 subunit
NCBI Official Symbol:	CHRNB2
NCBI Official Synonym Symbols:	EFNL3; nAChRB2
NCBI Protein Information:	neuronal acetylcholine receptor subunit beta-2
UniProt Protein Name:	Neuronal acetylcholine receptor subunit beta-2
Protein Family:	Neuronal acetylcholine receptor
UniProt Gene Name:	CHRNB2
UniProt Entry Name:	ACHB2_HUMAN

	Western blot analysis of extracts of various cell lines, using CHRNB2 antibody (CAB9808) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse brain using CHRNB2 antibody (CAB9808) at dilution of 1:100 (40x lens).