Immunology Antibodies 2

Anti-CHMP4B Antibody (CAB7402)

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SKU:
CAB7402
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-CHMP4B Antibody
Antibody SKU:CAB7402
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-224 of human CHMP4B (NP_789782.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:SH-SY5Y, HT-29, 293T, NCI-H460, THP-1, SKOV3, Mouse kidney, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-224 of human CHMP4B (NP_789782.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSVF GKLF GAGG GKAG KGGP TPQE AIQR LRDT EEML SKKQ EFLE KKIE QELT AAKK HGTK NKRA ALQA LKRK KRYE KQLA QIDG TLST IEFQ REAL ENAN TNTE VLKN MGYA AKAM KAAH DNMD IDKV DELM QDIA DQQE LAEE ISTA ISKP VGFG EEFD EDEL MAEL EELE QEEL DKNL LEIS GPET VPLP NVPS IALP SKPA KKKE EEDD DMKE LENW AGSM
Gene ID:128866
Uniprot:Q9H444
Cellular Location:Cytoplasm, Late endosome membrane, Peripheral membrane protein, cytosol
Calculated MW:24kDa
Observed MW:36kDa
Synonyms:CHMP4B, C20orf178, CHMP4A, CTPP3, CTRCT31, SNF7, SNF7-2, Shax1, VPS32B, Vps32-2, dJ553F4.4
Background:This gene encodes a member of the chromatin-modifying protein/charged multivesicular body protein (CHMP) protein family. The protein is part of the endosomal sorting complex required for transport (ESCRT) complex III (ESCRT-III), which functions in the sorting of endocytosed cell-surface receptors into multivesicular endosomes. The ESCRT machinery also functions in the final abscisson stage of cytokinesis and in the budding of enveloped viruses such as HIV-1. The three proteins of the CHMP4 subfamily interact with programmed cell death 6 interacting protein (PDCD6IP, also known as ALIX), which also functions in the ESCRT pathway. The CHMP4 proteins assemble into membrane-attached 5-nm filaments that form circular scaffolds and promote or stabilize outward budding. These polymers are proposed to help generate the luminal vesicles of multivesicular bodies. Mutations in this gene result in autosomal dominant posterior polar cataracts.
UniProt Protein Function:CHMP4B: Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. When overexpressed, membrane-assembled circular arrays of CHMP4B filaments can promote or stabilize negative curvature and outward budding. Via its interaction with PDCD6IP involved in HIV-1 p6- and p9-dependent virus release. Defects in CHMP4B are the cause of cataract posterior polar type 3 (CTPP3). A subcapsular opacity, usually disk-shaped, located at the back of the lens. It can have a marked effect on visual acuity. Belongs to the SNF7 family.
UniProt Protein Details:

Protein type:Membrane protein, peripheral

Chromosomal Location of Human Ortholog: 20q11.22

Cellular Component: internal side of plasma membrane; membrane coat; late endosome membrane; cytoplasm; midbody; nucleus; cytosol; endosome

Molecular Function:identical protein binding; protein binding; protein homodimerization activity

Biological Process: cell separation during cytokinesis; viral reproduction; vacuolar transport; non-lytic virus budding; regulation of viral reproduction; viral infectious cycle; posttranslational protein targeting to membrane; endosome transport; mitotic metaphase plate congression; nuclear organization and biogenesis; protein homooligomerization

Disease: Cataract 31, Multiple Types

NCBI Summary:This gene encodes a member of the chromatin-modifying protein/charged multivesicular body protein (CHMP) protein family. The protein is part of the endosomal sorting complex required for transport (ESCRT) complex III (ESCRT-III), which functions in the sorting of endocytosed cell-surface receptors into multivesicular endosomes. The ESCRT machinery also functions in the final abscisson stage of cytokinesis and in the budding of enveloped viruses such as HIV-1. The three proteins of the CHMP4 subfamily interact with programmed cell death 6 interacting protein (PDCD6IP, also known as ALIX), which also functions in the ESCRT pathway. The CHMP4 proteins assemble into membrane-attached 5-nm filaments that form circular scaffolds and promote or stabilize outward budding. These polymers are proposed to help generate the luminal vesicles of multivesicular bodies. Mutations in this gene result in autosomal dominant posterior polar cataracts.[provided by RefSeq, Oct 2009]
UniProt Code:Q9H444
NCBI GenInfo Identifier:24636296
NCBI Gene ID:128866
NCBI Accession:Q9H444.1
UniProt Secondary Accession:Q9H444,Q53ZD6, E1P5N4,
UniProt Related Accession:Q9H444
Molecular Weight:224
NCBI Full Name:Charged multivesicular body protein 4b
NCBI Synonym Full Names:charged multivesicular body protein 4B
NCBI Official Symbol:CHMP4B  
NCBI Official Synonym Symbols:SNF7; CTPP3; Shax1; CHMP4A; SNF7-2; VPS32B; CTRCT31; Vps32-2; C20orf178; dJ553F4.4  
NCBI Protein Information:charged multivesicular body protein 4b; hSnf7-2; hVps32-2; chromatin modifying protein 4B; chromatin-modifying protein 4b; SNF7 homolog associated with Alix 1; Snf7 homologue associated with Alix 1; vacuolar protein sorting-associated protein 32-2; vacuolar protein-sorting-associated protein 32-2
UniProt Protein Name:Charged multivesicular body protein 4b
UniProt Synonym Protein Names:Chromatin-modifying protein 4b; CHMP4b; SNF7 homolog associated with Alix 1; SNF7-2; hSnf7-2; Vacuolar protein sorting-associated protein 32-2; Vps32-2; hVps32-2
Protein Family:Charged multivesicular body protein
UniProt Gene Name:CHMP4B  
UniProt Entry Name:CHM4B_HUMAN
Anti-CHMP4B Antibody (CAB7402)Western blot analysis of extracts of various cell lines, using CHMP4B antibody (CAB7402) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 20s.
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