Metabolism Antibodies 2

CHIT1 Rabbit Polyclonal Antibody (CAB2015)

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SKU:
CAB2015
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse
Synonyms:
CHI3, CHIT, CHITD, CHIT1
Research Use:
Metabolism
Frequently bought together:

Description

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CHIT1 Antibody (CAB2015)

The CHIT1 Antibody (CAB2015) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific to human chitotriosidase-1 and has been validated for use in various applications, including Western blotting. Chitotriosidase-1 is known to play a role in the immune system, particularly in the defense against chitin-containing pathogens. It is also implicated in various diseases, such as Gaucher disease, where its levels are increased.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:CHIT1 Antibody
SKU:CAB2015
Size:20μL, 100μL
Reactivity:Human, Mouse
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:SWEK VTGH NSPL YKRQ EESG AAAS LNVD AAVQ QWLQ KGTP ASKL ILGM PTYG RSFT LASS SDTR VGAP ATGS GTPG PFTK EGGM LAYY EVCS WKGA TKQR IQDQ KVPY IFRD NQWV GFDD VESF KTKV SYLK QKGL GGAM VWAL DLDD FAGF SCNQ GRYP LIQT LRQE LSLP YLPS GTPE LEVP KPGQ PSEP EHGP SPGQ DTFC QGKA DGLY PNPR ERSS FYSC AAGR LFQQ SCPT GLVF SNSC KCCT WN
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:500 - 1:5000
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CHI3, CHIT, CHITD, CHIT1
Positive Sample:U-251MG, 293T, Mouse spleen, Mouse skeletal muscle
Cellular Localization:Lysosome, Secreted.
Calculated MW:52kDa
Observed MW:52kDa

Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene.

Purification MethodAffinity purification
Gene ID1118
RRIDAB_2764039
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using CHIT1 Rabbit pAb (CAB2015) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human normal stomach using CHIT1 Rabbit pAb (CAB2015) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
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