Epigenetics & Nuclear Signaling Antibodies 1
Anti-CHD9 Antibody (CAB12147)
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- SKU:
- CAB12147
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-CHD9 Antibody |
| Antibody SKU: | CAB12147 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 85-190 of human CHD9 (NP_079410.4). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | U-87MG, HeLa, Jurkat, A-549, 293T, K-562, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 85-190 of human CHD9 (NP_079410.4). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SFGS PAEH VLSP HSQF NCSP IHPQ NQPN GLFP DVSD GSPM WGHQ TATT ISNQ NGSP FHQQ GHSH SMHQ NKSF VAHH DFAL FQAN EQQT QCTS LRSQ QNRN NLNP GQ |
| Gene ID: | 80205 |
| Uniprot: | Q3L8U1 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 324kDa/326kDa |
| Observed MW: | 300kDa |
| Synonyms: | CHD9, AD013, CHD-9, CReMM, KISH2, PRIC320 |
| Background: |
| UniProt Protein Function: | CHD-9: Acts as a transcriptional coactivator for PPARA and possibly other nuclear receptors. Proposed to be a ATP-dependent chromatin remodeling protein. Has DNA-dependent ATPase activity and binds to A/T-rich DNA. Associates with A/T-rich regulatory regions in promoters of genes that participate in the differentiation of progenitors during osteogenesis. Belongs to the SNF2/RAD54 helicase family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA-binding; EC 3.6.4.12; Helicase Chromosomal Location of Human Ortholog: 16q12.2 Cellular Component: nucleoplasm Biological Process: cellular lipid metabolic process |
| UniProt Code: | Q3L8U1 |
| NCBI GenInfo Identifier: | 215273951 |
| NCBI Gene ID: | 80205 |
| NCBI Accession: | Q3L8U1.2 |
| UniProt Secondary Accession: | Q3L8U1,O15025, Q1WF12, Q6DTK9, Q9H9V7, Q9UHM2, B2RTU2 B9ZVQ0, |
| UniProt Related Accession: | Q3L8U1 |
| Molecular Weight: | 326kDa |
| NCBI Full Name: | Chromodomain-helicase-DNA-binding protein 9 |
| NCBI Synonym Full Names: | chromodomain helicase DNA binding protein 9 |
| NCBI Official Symbol: | CHD9 |
| NCBI Official Synonym Symbols: | AD013; CHD-9; CReMM; KISH2; PRIC320 |
| NCBI Protein Information: | chromodomain-helicase-DNA-binding protein 9 |
| UniProt Protein Name: | Chromodomain-helicase-DNA-binding protein 9 |
| UniProt Synonym Protein Names: | ATP-dependent helicase CHD9; Chromatin-related mesenchymal modulator; CReMM; Chromatin-remodeling factor CHROM1; Kismet homolog 2; PPAR-alpha-interacting complex protein 320 kDa; Peroxisomal proliferator-activated receptor A-interacting complex 320 kDa protein |
| Protein Family: | Chromodomain-helicase-DNA-binding protein |
| UniProt Gene Name: | CHD9 |
 | Western blot analysis of extracts of various cell lines, using CHD9 antibody (CAB12147) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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