Epigenetics & Nuclear Signaling Antibodies 1

Anti-CHD9 Antibody (CAB12147)

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SKU:
CAB12147
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-CHD9 Antibody
Antibody SKU:CAB12147
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 85-190 of human CHD9 (NP_079410.4).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:U-87MG, HeLa, Jurkat, A-549, 293T, K-562, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 85-190 of human CHD9 (NP_079410.4).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SFGS PAEH VLSP HSQF NCSP IHPQ NQPN GLFP DVSD GSPM WGHQ TATT ISNQ NGSP FHQQ GHSH SMHQ NKSF VAHH DFAL FQAN EQQT QCTS LRSQ QNRN NLNP GQ
Gene ID:80205
Uniprot:Q3L8U1
Cellular Location:Cytoplasm, Nucleus
Calculated MW:324kDa/326kDa
Observed MW:300kDa
Synonyms:CHD9, AD013, CHD-9, CReMM, KISH2, PRIC320
Background:
UniProt Protein Function:CHD-9: Acts as a transcriptional coactivator for PPARA and possibly other nuclear receptors. Proposed to be a ATP-dependent chromatin remodeling protein. Has DNA-dependent ATPase activity and binds to A/T-rich DNA. Associates with A/T-rich regulatory regions in promoters of genes that participate in the differentiation of progenitors during osteogenesis. Belongs to the SNF2/RAD54 helicase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA-binding; EC 3.6.4.12; Helicase

Chromosomal Location of Human Ortholog: 16q12.2

Cellular Component: nucleoplasm

Biological Process: cellular lipid metabolic process

UniProt Code:Q3L8U1
NCBI GenInfo Identifier:215273951
NCBI Gene ID:80205
NCBI Accession:Q3L8U1.2
UniProt Secondary Accession:Q3L8U1,O15025, Q1WF12, Q6DTK9, Q9H9V7, Q9UHM2, B2RTU2 B9ZVQ0,
UniProt Related Accession:Q3L8U1
Molecular Weight:326kDa
NCBI Full Name:Chromodomain-helicase-DNA-binding protein 9
NCBI Synonym Full Names:chromodomain helicase DNA binding protein 9
NCBI Official Symbol:CHD9  
NCBI Official Synonym Symbols:AD013; CHD-9; CReMM; KISH2; PRIC320  
NCBI Protein Information:chromodomain-helicase-DNA-binding protein 9
UniProt Protein Name:Chromodomain-helicase-DNA-binding protein 9
UniProt Synonym Protein Names:ATP-dependent helicase CHD9; Chromatin-related mesenchymal modulator; CReMM; Chromatin-remodeling factor CHROM1; Kismet homolog 2; PPAR-alpha-interacting complex protein 320 kDa; Peroxisomal proliferator-activated receptor A-interacting complex 320 kDa protein
Protein Family:Chromodomain-helicase-DNA-binding protein
UniProt Gene Name:CHD9  
Anti-CHD9 Antibody (CAB12147)Western blot analysis of extracts of various cell lines, using CHD9 antibody (CAB12147) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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