Epigenetics & Nuclear Signaling Antibodies 1
Anti-CHD4 Antibody (CAB11574)
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- SKU:
- CAB11574
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-CHD4 Antibody |
| Antibody SKU: | CAB11574 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1520-1690 of human CHD4 (NP_001264.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, Jurkat, 293T, HT-1080 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1520-1690 of human CHD4 (NP_001264.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ELAE VEEN KKMS QPGS PSPK TPTP STPG DTQP NTPA PVPP AEDG IKIE ENSL KEEE SIEG EKEV KSTA PETA IECT QAPA PASE DEKV VVEP PEGE EKVE KAEV KERT EEPM ETEP KGAA DVEK VEEK SAID LTPI VVED KEEK KEEE EKKE VMLQ NGET PKDL NDEK QKK |
| Gene ID: | 1108 |
| Uniprot: | Q14839 |
| Cellular Location: | Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center |
| Calculated MW: | 218kDa/220kDa |
| Observed MW: | 280kDa |
| Synonyms: | CHD4, CHD-4, Mi-2b, Mi2-BETA, SIHIWES |
| Background: | The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| UniProt Protein Function: | Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. |
| NCBI Summary: | The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014] |
| UniProt Code: | Q14839 |
| NCBI GenInfo Identifier: | 311033360 |
| NCBI Gene ID: | 1108 |
| NCBI Accession: | Q14839.2 |
| UniProt Secondary Accession: | Q14839,Q8IXZ5, |
| UniProt Related Accession: | Q14839 |
| Molecular Weight: | 278 kDa |
| NCBI Full Name: | Chromodomain-helicase-DNA-binding protein 4 |
| NCBI Synonym Full Names: | chromodomain helicase DNA binding protein 4 |
| NCBI Official Symbol: | CHD4 |
| NCBI Official Synonym Symbols: | CHD-4; Mi-2b; SIHIWES; Mi2-BETA |
| NCBI Protein Information: | chromodomain-helicase-DNA-binding protein 4 |
| UniProt Protein Name: | Chromodomain-helicase-DNA-binding protein 4 |
| UniProt Synonym Protein Names: | ATP-dependent helicase CHD4; Mi-2 autoantigen 218 kDa protein; Mi2-beta |
| Protein Family: | Chromodomain-helicase-DNA-binding protein |
| UniProt Gene Name: | CHD4 |
 | Western blot analysis of extracts of various cell lines, using CHD4 antibody (CAB11574) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded Rat liver using CHD4 antibody (CAB11574) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Human oophoroma using CHD4 antibody (CAB11574) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Mouse liver using CHD4 antibody (CAB11574) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using CHD4 antibody (CAB11574) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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