Cardiovascular Antibodies

Anti-CHD2 Antibody (CAB5895)

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SKU:
CAB5895
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cardiovascular

Description

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Antibody Name:Anti-CHD2 Antibody
Antibody SKU:CAB5895
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-223 of human CHD2 (NP_001036037.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat, DU145, SW620, Mouse lung, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-223 of human CHD2 (NP_001036037.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MMRN KDKS QEED SSLH SNAS SHSA SEEA SGSD SGSQ SESE QGSD PGSG HGSE SNSS SESS ESQS ESES ESAG SKSQ PVLP EAKE KPAS KKER IADV KKMW EEYP DVYG VRRS NRSR QEPS RFNI KEEA SSGS ESGS PKRR GQRQ LKKQ EKWK QEPS EDEQ EQGT SAES EPEQ KKVK ARRP VPRR TVPK PRVK KQPK TQRG KRKK QDSS DEDD DDDE APKR QTR
Gene ID:1106
Uniprot:O14647
Cellular Location:Nucleus
Calculated MW:56kDa/200kDa/211kDa
Observed MW:260kDa
Synonyms:CHD2, EEOC
Background:The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
UniProt Protein Function:CHD-2: Sequence-selective DNA-binding protein. Belongs to the SNF2/RAD54 helicase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.6.4.12; Helicase; DNA-binding

Chromosomal Location of Human Ortholog: 15q26

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; nucleolus; nucleus

Molecular Function:ATP-dependent DNA helicase activity; DNA binding; histone binding; ATP binding

Biological Process: regulation of transcription from RNA polymerase II promoter; muscle development; transcription, DNA-dependent; chromatin modification; response to DNA damage stimulus; DNA duplex unwinding

Disease: Epileptic Encephalopathy, Childhood-onset

NCBI Summary:The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:O14647
NCBI GenInfo Identifier:119370320
NCBI Gene ID:1106
NCBI Accession:O14647.2
UniProt Secondary Accession:O14647,Q96IP5, C6G482,
UniProt Related Accession:O14647
Molecular Weight:1828
NCBI Full Name:Chromodomain-helicase-DNA-binding protein 2
NCBI Synonym Full Names:chromodomain helicase DNA binding protein 2
NCBI Official Symbol:CHD2  
NCBI Official Synonym Symbols:EEOC  
NCBI Protein Information:chromodomain-helicase-DNA-binding protein 2; CHD-2; ATP-dependent helicase CHD2
UniProt Protein Name:Chromodomain-helicase-DNA-binding protein 2
UniProt Synonym Protein Names:ATP-dependent helicase CHD2
Protein Family:Chromodomain-helicase-DNA-binding protein
UniProt Gene Name:CHD2  
UniProt Entry Name:CHD2_HUMAN
Anti-CHD2 Antibody (CAB5895)Western blot analysis of extracts of various cell lines, using CHD2 antibody (CAB5895) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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