KO Validated Antibodies 1

[KO Validated] CHD1 Rabbit Polyclonal Antibody (CAB7883)

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SKU:
CAB7883
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse, Rat
Synonyms:
CHD-1, PILBOS, D1
Research Use:
Epigenetics and Nuclear Signaling
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Description

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[KO Validated] CHD1 Antibody (CAB7883)

The [KO Validated] CHD1 Antibody (CAB7883) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.The CHD1 protein is involved in modulating chromatin structure, which in turn affects gene transcription and cellular processes. By targeting the CHD1 protein, researchers can gain insight into how chromatin remodeling contributes to various biological functions, including cell differentiation, development, and disease progression.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] CHD1 Antibody
SKU:CAB7883
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:DREK HRKL DDHR SRDH RSNL EGSL KDRS HSDH RSHS DHRL HSDH RSSS EYTH HKSS RDYR YHSD WQMD HRAS SSGP RSPL DQRS PYGS RSPF EHSV EHKS TPEH TWSS RKT
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:100 - 1:500
IHC-P1:100 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CHD-1, PILBOS, D1
Positive Sample:Jurkat
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:197kDa
Observed MW:250kDa

The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template.

Purification MethodAffinity purification
Gene ID1105
RRIDAB_2863563
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and CHD1 knockout (KO) 293T cells, using [KO Validated] CHD1 Rabbit pAb (CAB7883) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageWestern blot analysis of lysates from Jurkat cells, using [KO Validated] CHD1 Rabbit pAb (CAB7883) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human liver damage using [KO Validated] CHD1 Rabbit pAb (CAB7883) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat brain using [KO Validated] CHD1 Rabbit pAb (CAB7883) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
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