Anti-CHD1 Antibody (CAB7883)[KO Validated]

Name: Anti-CHD1 Antibody (CAB7883)[KO Validated]
Brand: KO Validated Antibodies 1
SKU: CAB7883
Availability: OutOfStock

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KO Validated Antibodies 2

KO Validated Antibodies 1

KO Validated Antibodies 1

KO Validated Antibodies 1

KO Validated Antibodies 1

Antibody Name:	Anti-CHD1 Antibody [KO Validated]
Antibody SKU:	CAB7883
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1600 to the C-terminus of human CHD1 (NP_001261.2).

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1600 to the C-terminus of human CHD1 (NP_001261.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DREK HRKL DDHR SRDH RSNL EGSL KDRS HSDH RSHS DHRL HSDH RSSS EYTH HKSS RDYR YHSD WQMD HRAS SSGP RSPL DQRS PYGS RSPF EHSV EHKS TPEH TWSS RKT
Gene ID:	1105
Uniprot:	O14646
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	196kDa
Observed MW:	197kDa

Synonyms:	CHD1
Background:	The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template.

UniProt Protein Function:	CHD-1: a sequence-selective DNA-binding protein. Contains two chromo (chromatin organization modifier) domains and one SNF2-related helicase and ATPase domains. May play an important role in gene regulation.
UniProt Protein Details:	Protein type:Helicase; EC 3.6.4.12 Chromosomal Location of Human Ortholog: 5q15-q21 Cellular Component: cytoplasm; nucleus Molecular Function:ATP-dependent DNA helicase activity; protein binding; DNA binding; methylated histone residue binding; ATP binding Biological Process: chromatin remodeling; regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; DNA duplex unwinding
UniProt Code:	O14646
NCBI GenInfo Identifier:	269849549
NCBI Gene ID:
NCBI Accession:	O14646.2
Molecular Weight:	197kDa
NCBI Full Name:	Chromodomain-helicase-DNA-binding protein 1
UniProt Protein Name:	Chromodomain-helicase-DNA-binding protein 1
UniProt Synonym Protein Names:	ATP-dependent helicase CHD1
Protein Family:	Chromodomain-helicase-DNA-binding protein
UniProt Gene Name:	CHD1
UniProt Entry Name:	CHD1_HUMAN

	Western blot analysis of extracts of various cell lines, using CHD1 Antibody (CAB7883) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of extracts from normal (control) and CHD1 knockout (KO) 293T cells, using CHD1 antibody (CAB7883) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse brain using CHD1 antibody (CAB7883) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver damage using CHD1 antibody (CAB7883) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using CHD1 antibody (CAB7883) at dilution of 1:100 (40x lens).