KO Validated Antibodies 1

Anti-CHD1 Antibody (CAB7883)[KO Validated]

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SKU:
CAB7883
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-CHD1 Antibody [KO Validated]
Antibody SKU:CAB7883
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1600 to the C-terminus of human CHD1 (NP_001261.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, Jurkat, K-562
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1600 to the C-terminus of human CHD1 (NP_001261.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:DREK HRKL DDHR SRDH RSNL EGSL KDRS HSDH RSHS DHRL HSDH RSSS EYTH HKSS RDYR YHSD WQMD HRAS SSGP RSPL DQRS PYGS RSPF EHSV EHKS TPEH TWSS RKT
Gene ID:1105
Uniprot:O14646
Cellular Location:Cytoplasm, Nucleus
Calculated MW:196kDa
Observed MW:197kDa
Synonyms:CHD1
Background:The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template.
UniProt Protein Function:CHD-1: a sequence-selective DNA-binding protein. Contains two chromo (chromatin organization modifier) domains and one SNF2-related helicase and ATPase domains. May play an important role in gene regulation.
UniProt Protein Details:

Protein type:Helicase; EC 3.6.4.12

Chromosomal Location of Human Ortholog: 5q15-q21

Cellular Component: cytoplasm; nucleus

Molecular Function:ATP-dependent DNA helicase activity; protein binding; DNA binding; methylated histone residue binding; ATP binding

Biological Process: chromatin remodeling; regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; DNA duplex unwinding

UniProt Code:O14646
NCBI GenInfo Identifier:269849549
NCBI Gene ID:
NCBI Accession:O14646.2
Molecular Weight:197kDa
NCBI Full Name:Chromodomain-helicase-DNA-binding protein 1
UniProt Protein Name:Chromodomain-helicase-DNA-binding protein 1
UniProt Synonym Protein Names:ATP-dependent helicase CHD1
Protein Family:Chromodomain-helicase-DNA-binding protein
UniProt Gene Name:CHD1  
UniProt Entry Name:CHD1_HUMAN
Anti-CHD1 Antibody [KO Validated] (CAB7883)Western blot analysis of extracts of various cell lines, using CHD1 Antibody (CAB7883) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-CHD1 Antibody [KO Validated] (CAB7883)Western blot analysis of extracts from normal (control) and CHD1 knockout (KO) 293T cells, using CHD1 antibody (CAB7883) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-CHD1 Antibody [KO Validated] (CAB7883)Immunohistochemistry of paraffin-embedded mouse brain using CHD1 antibody (CAB7883) at dilution of 1:100 (40x lens).
Anti-CHD1 Antibody [KO Validated] (CAB7883)Immunohistochemistry of paraffin-embedded human liver damage using CHD1 antibody (CAB7883) at dilution of 1:100 (40x lens).
Anti-CHD1 Antibody [KO Validated] (CAB7883)Immunohistochemistry of paraffin-embedded rat brain using CHD1 antibody (CAB7883) at dilution of 1:100 (40x lens).
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