KO Validated Antibodies 1

Anti-CHCHD2 Antibody (CAB16645)[KO Validated]

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SKU:
CAB16645
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-CHCHD2 Antibody [KO Validated]
Antibody SKU:CAB16645
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 75-145 of human CHCHD2 (NP_057223.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HT-29, 293T, HepG2, U-87MG, mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 75-145 of human CHCHD2 (NP_057223.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TLGH AITG GFSG GSNA EPAR PDIT YQEP QGTQ PAQQ QQPC LYEI KQFL ECAQ NQGD IKLC EGFN EVLK QCR
Gene ID:51142
Uniprot:Q9Y6H1
Cellular Location:Nucleus
Calculated MW:15kDa
Observed MW:16kDa
Synonyms:CHCHD2, C7orf17, MNRR1, NS2TP, PARK22
Background:The protein encoded by this gene belongs to a class of eukaryotic CX(9)C proteins characterized by four cysteine residues spaced ten amino acids apart from one another. These residues form disulfide linkages that define a CHCH fold. In response to stress, the protein translocates from the mitochondrial intermembrane space to the nucleus where it binds to a highly conserved 13 nucleotide oxygen responsive element in the promoter of cytochrome oxidase 4I2, a subunit of the terminal enzyme of the electron transport chain. In concert with recombination signal sequence-binding protein J, binding of this protein activates the oxygen responsive element at four percent oxygen. In addition, it has been shown that this protein is a negative regulator of mitochondria-mediated apoptosis. In response to apoptotic stimuli, mitochondrial levels of this protein decrease, allowing BCL2-associated X protein to oligomerize and activate the caspase cascade. Pseudogenes of this gene are found on multiple chromosomes. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:CHCHD2: Transcription factor. Binds to the oxygen responsive element of COX4I2 and activates its transcription under hypoxia conditions (4% oxygen), as well as normoxia conditions (20% oxygen) (PubMed:23303788). {ECO:0000269|PubMed:23303788}. Up-regulated by hypoxia (4% oxygen) (at protein level). {ECO:0000269|PubMed:23303788}. Interacts with RBPJ. {ECO:0000269|PubMed:23303788}
UniProt Protein Details:

Protein type:Unknown function

Chromosomal Location of Human Ortholog: 7p11.2

Cellular Component: mitochondrion

UniProt Code:Q9Y6H1
NCBI GenInfo Identifier:7705851
NCBI Gene ID:51142
NCBI Accession:NP_057223.1
UniProt Secondary Accession:Q9Y6H1,Q498C3, Q6NZ50,
UniProt Related Accession:Q9Y6H1
Molecular Weight:15,513 Da
NCBI Full Name:coiled-coil-helix-coiled-coil-helix domain-containing protein 2, mitochondrial
NCBI Synonym Full Names:coiled-coil-helix-coiled-coil-helix domain containing 2
NCBI Official Symbol:CHCHD2  
NCBI Official Synonym Symbols:C7orf17  
NCBI Protein Information:coiled-coil-helix-coiled-coil-helix domain-containing protein 2, mitochondrial; 16.7kD protein; HCV NS2 trans-regulated protein; NS2TP; aging-associated gene 10 protein
UniProt Protein Name:Coiled-coil-helix-coiled-coil-helix domain-containing protein 2, mitochondrial
UniProt Synonym Protein Names:Aging-associated gene 10 protein; HCV NS2 trans-regulated protein; NS2TP
Protein Family:Coiled-coil-helix-coiled-coil-helix domain-containing protein
UniProt Gene Name:CHCHD2  
UniProt Entry Name:CHCH2_HUMAN
Anti-CHCHD2 Antibody [KO Validated] (CAB16645)Western blot analysis of extracts of various cell lines, using CHCHD2 antibody (CAB16645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-CHCHD2 Antibody [KO Validated] (CAB16645)Immunohistochemistry of paraffin-embedded Mouse kidney using [KO Validated] CHCHD2 Rabbit pAb (CAB16645) at dilution of 1:100 (40x lens).
Anti-CHCHD2 Antibody [KO Validated] (CAB16645)Immunohistochemistry of paraffin-embedded Rat ovary using [KO Validated] CHCHD2 Rabbit pAb (CAB16645) at dilution of 1:100 (40x lens).
Anti-CHCHD2 Antibody [KO Validated] (CAB16645)Immunohistochemistry of paraffin-embedded Human colon carcinoma using [KO Validated] CHCHD2 Rabbit pAb (CAB16645) at dilution of 1:100 (40x lens).
Anti-CHCHD2 Antibody [KO Validated] (CAB16645)Immunofluorescence analysis of C6 cells using CHCHD2 antibody (CAB16645) at dilution of 1:100. Blue: DAPI for nuclear staining.
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