Cell Biology Antibodies 6
Anti-CGN Antibody (CAB15489)
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- SKU:
- CAB15489
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-CGN Antibody |
| Antibody SKU: | CAB15489 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 904-1203 of human CGN (NP_065821.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:200 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: | BxPC-3, 293T, LO2, A-549 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 904-1203 of human CGN (NP_065821.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TSGG LSRL QDEI QRLR QALQ ASQA ERDT ARLD KELL AQRL QGLE QEAE NKKR SQDD RARQ LKGL EEKV SRLE TELD EEKN TVEL LTDR VNRG RDQV DQLR TELM QERS ARQD LECD KISL ERQN KDLK TRLA SSEG FQKP SASL SQLE SQNQ LLQE RLQA EERE KTVL QSTN RKLE RKVK ELSI QIED ERQH VNDQ KDQL SLRV KALK RQVD EAEE EIER LDGL RKKA QREV EEQH EVNE QLQA RIKS LEKD SWRK ASRS AAES ALKN EGLS SDEE FDSV YDPS SIAS LLTE SNLQ TSSC |
| Gene ID: | 57530 |
| Uniprot: | Q9P2M7 |
| Cellular Location: | Cell junction, tight junction |
| Calculated MW: | 87kDa/136kDa |
| Observed MW: | 160kDa |
| Synonyms: | CGN, cingulin |
| Background: |
| UniProt Protein Function: | CGN: Probably plays a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. Belongs to the cingulin family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 1q21 Cellular Component: tight junction; myosin complex; cell junction Molecular Function:protein binding; microtubule binding; motor activity; actin binding Biological Process: metabolic process; transforming growth factor beta receptor signaling pathway; microtubule cytoskeleton organization and biogenesis |
| UniProt Code: | Q9P2M7 |
| NCBI GenInfo Identifier: | 16262452 |
| NCBI Gene ID: | 57530 |
| NCBI Accession: | NP_065821.1 |
| UniProt Secondary Accession: | Q9P2M7,Q5T386, Q9NR25, A6H8L3, A7MD22, |
| UniProt Related Accession: | Q9P2M7 |
| Molecular Weight: | |
| NCBI Full Name: | cingulin |
| NCBI Synonym Full Names: | cingulin |
| NCBI Official Symbol: | CGN |
| NCBI Protein Information: | cingulin |
| UniProt Protein Name: | Cingulin |
| Protein Family: | Cingulin |
| UniProt Gene Name: | CGN |
| UniProt Entry Name: | CING_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CGN antibody (CAB15489) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded Human colon carcinoma using CGN Rabbit pAb (CAB15489) at dilution of 1:100 (40x lens). |
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