Immunology Antibodies 2
Anti-CFP Antibody (CAB5398)
- SKU:
- CAB5398
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-CFP Antibody |
| Antibody SKU: | CAB5398 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 190-469 of human CFP (NP_001138724.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Mouse spleen, Jurkat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 190-469 of human CFP (NP_001138724.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | CPTH GAWA TWGP WTPC SASC HGGP HEPK ETRS RKCS APEP SQKP PGKP CPGL AYEQ RRCT GLPP CPVA GGWG PWGP VSPC PVTC GLGQ TMEQ RTCN HPVP QHGG PFCA GDAT RTHI CNTA VPCP VDGE WDSW GEWS PCIR RNMK SISC QEIP GQQS RGRT CRGR KFDG HRCA GQQQ DIRH CYSI QHCP LKGS WSEW STWG LCMP PCGP NPTR ARQR LCTP LLPK YPPT VSMV EGQG EKNV TFWG RPLP RCEE LQGQ KLVV EEKR PCLH VPAC KDPE EEEL |
| Gene ID: | 5199 |
| Uniprot: | P27918 |
| Cellular Location: | Secreted |
| Calculated MW: | 51kDa |
| Observed MW: | 50kDa |
| Synonyms: | CFP, BFD, PFC, PFD, PROPERDIN, properdin |
| Background: | This gene encodes a plasma glycoprotein that positively regulates the alternative complement pathway of the innate immune system. This protein binds to many microbial surfaces and apoptotic cells and stabilizes the C3- and C5-convertase enzyme complexes in a feedback loop that ultimately leads to formation of the membrane attack complex and lysis of the target cell. Mutations in this gene result in two forms of properdin deficiency, which results in high susceptibility to meningococcal infections. Multiple alternatively spliced variants, encoding the same protein, have been identified. |
| UniProt Protein Function: | CFP: A positive regulator of the alternate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes. Defects in CFP are the cause of properdin deficiency (PFD). PFD results in higher susceptibility to bacterial infections; especially to meningococcal infections. Three phenotypes have been reported: complete deficiency (type I), incomplete deficiency (type II), and dysfunction of properdin (type III). |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: Xp11.4 Cellular Component: extracellular matrix; extracellular space; endoplasmic reticulum lumen; extracellular region Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; defense response to bacterium; complement activation, alternative pathway; regulation of complement activation; innate immune response; immune response; post-translational protein modification; complement activation Disease: Properdin Deficiency, X-linked |
| NCBI Summary: | This gene encodes a plasma glycoprotein that positively regulates the alternative complement pathway of the innate immune system. This protein binds to many microbial surfaces and apoptotic cells and stabilizes the C3- and C5-convertase enzyme complexes in a feedback loop that ultimately leads to formation of the membrane attack complex and lysis of the target cell. Mutations in this gene result in two forms of properdin deficiency, which results in high susceptibility to meningococcal infections. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Feb 2009] |
| UniProt Code: | P27918 |
| NCBI GenInfo Identifier: | 464473 |
| NCBI Gene ID: | 5199 |
| NCBI Accession: | P27918.2 |
| UniProt Secondary Accession: | P27918,O15134, O15135, O15136, O75826, |
| UniProt Related Accession: | P27918 |
| Molecular Weight: | 469 |
| NCBI Full Name: | Properdin |
| NCBI Synonym Full Names: | complement factor properdin |
| NCBI Official Symbol: | CFP |
| NCBI Official Synonym Symbols: | BFD; PFC; PFD; PROPERDIN |
| NCBI Protein Information: | properdin; complement factor P; properdin P factor, complement |
| UniProt Protein Name: | Properdin |
| UniProt Synonym Protein Names: | Complement factor P |
| Protein Family: | Properdin |
| UniProt Gene Name: | CFP |
| UniProt Entry Name: | PROP_HUMAN |
 | Western blot analysis of extracts of mouse spleen, using CFP antibody (CAB5398) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Western blot analysis of extracts of Jurkat cells, using CFP antibody (CAB5398) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of NIH-3T3 cells using CFP Rabbit pAb (CAB5398) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using CFP Rabbit pAb (CAB5398) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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