Cell Death Antibodies 1

Anti-cFLIP Antibody (CAB2555)

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SKU:
CAB2555
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death

Description

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Antibody Name:Anti-cFLIP Antibody
Antibody SKU:CAB2555
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 161-480 of human cFLIP (NP_001120655.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat, Mouse liver, Mouse heart, Rat liver, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 161-480 of human cFLIP (NP_001120655.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:RIDL KTKI QKYK QSVQ GAGT SYRN VLQA AIQK SLKD PSNN FRLH NGRS KEQR LKEQ LGAQ QEPV KKSI QESE AFLP QSIP EERY KMKS KPLG ICLI IDCI GNET ELLR DTFT SLGY EVQK FLHL SMHG ISQI LGQF ACMP EHRD YDSF VCVL VSRG GSQS VYGV DQTH SGLP LHHI RRMF MGDS CPYL AGKP KMFF IQNY VVSE GQLE DSSL LEVD GPAM KNVE FKAQ KRGL CTVH READ FFWS LCTA DMSL LEQS HSSP SLYL QCLS QKLR QERK RPLL DLHI ELNG YMYD WNSR VSAK EKYY VWLQ HTLR KKLI LSYT
Gene ID:8837
Uniprot:O15519
Cellular Location:
Calculated MW:23-55kDa
Observed MW:28kDa/55kDa
Synonyms:CFLAR, CASH, CASP8AP1, CLARP, Casper, FLAME, FLAME-1, FLAME1, FLIP, I-FLICE, MRIT, c-FLIP, c-FLIPL, c-FLIPR, c-FLIPS
Background:The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists.
NCBI Summary:The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011]
UniProt Code:O15519
NCBI GenInfo Identifier:12643547
NCBI Gene ID:8837
NCBI Accession:O15519.1
UniProt Related Accession:O15519
Molecular Weight:~ 55kDa
NCBI Full Name:CASP8 and FADD-like apoptosis regulator
NCBI Synonym Full Names:CASP8 and FADD like apoptosis regulator
NCBI Official Symbol:CFLAR  
NCBI Official Synonym Symbols:CASH; FLIP; MRIT; CLARP; FLAME; cFLIP; Casper; FLAME1; c-FLIP; FLAME-1; I-FLICE; c-FLIPL; c-FLIPR; c-FLIPS; CASP8AP1  
NCBI Protein Information:CASP8 and FADD-like apoptosis regulator
UniProt Protein Name:CASP8 and FADD-like apoptosis regulator
UniProt Synonym Protein Names:Caspase homolog; CASH; Caspase-eight-related protein; Casper; Caspase-like apoptosis regulatory protein; CLARP; Cellular FLICE-like inhibitory protein; c-FLIP; FADD-like antiapoptotic molecule 1; FLAME-1; Inhibitor of FLICE; I-FLICE; MACH-related inducer of toxicity; MRIT; UsurpinCleaved into the following 2 chains:CASP8 and FADD-like apoptosis regulator subunit p43; CASP8 and FADD-like apoptosis regulator subunit p12
Protein Family:Flippase kinase
UniProt Gene Name:CFLAR  
UniProt Entry Name:CFLAR_HUMAN
Anti-cFLIP Antibody (CAB2555)Western blot analysis of extracts of Jurkat cells, using cFLIP antibody (CAB2555) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-cFLIP Antibody (CAB2555)Western blot analysis of extracts of various cell lines, using cFLIP antibody (CAB2555) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-cFLIP Antibody (CAB2555)Immunohistochemistry of paraffin-embedded human thyroid cancer using cFLIP antibody (CAB2555) at dilution of 1:100 (40x lens).
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