Epigenetics & Nuclear Signaling Antibodies 2
Anti-CEP85 Antibody (CAB15891)
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- SKU:
- CAB15891
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-CEP85 Antibody |
| Antibody SKU: | CAB15891 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 130-430 of human CEP85 (NP_073615.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-431, Jurkat, HeLa, |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 130-430 of human CEP85 (NP_073615.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TRNG DLGA MKHS PGLS RDLM YFSG ATGE NGIE QSWF PAVG HERQ EEAR KFDI PSME STLN QSAM METL YSDP HHRV RFHN PRTS TSKE LYRV LPEA KKAP GSGA VFER NGPH SNSS GVLP LGLQ PAPG LSKP LPSQ VWQP SPDT WHPR EQSC ELST CRQQ LELI RLQM EQMQ LQNG AICH HPAA FGPS LPIL EPAQ WISI LNSN EHLL KEKE LLID KQRK HISQ LEQK VRES ELQV HSAL LGRP APFG DVCL LRLQ ELQR ENTF LRAQ FAQK TEAL SREK IDLE KKLS ASEV EVQL IRES L |
| Gene ID: | 64793 |
| Uniprot: | Q6P2H3 |
| Cellular Location: | Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center, nucleolus, spindle pole |
| Calculated MW: | 22kDa/80kDa/85kDa |
| Observed MW: | 105kDa |
| Synonyms: | CEP85, CCDC21 |
| Background: | This gene encodes a protein that belongs to the centrosome-associated family of proteins. The centrosome is a subcellular organelle in the animal cell that functions as a microtubule organizing center and is involved in cell-cycle progression. Alternate splicing results in multiple transcript variants. |
| UniProt Protein Function: | CCDC21: Belongs to the CEP85 family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cytoskeletal; Nucleolus Chromosomal Location of Human Ortholog: 1p36.11 Cellular Component: Golgi apparatus; spindle pole; centrosome; cytoplasm; nucleolus Molecular Function:protein binding |
| NCBI Summary: | This gene encodes a protein that belongs to the centrosome-associated family of proteins. The centrosome is a subcellular organelle in the animal cell that functions as a microtubule organizing center and is involved in cell-cycle progression. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
| UniProt Code: | Q6P2H3 |
| NCBI GenInfo Identifier: | 528078329 |
| NCBI Gene ID: | 64793 |
| NCBI Accession: | NP_001268446.1 |
| UniProt Secondary Accession: | Q6P2H3,Q5VY68, Q5VY70, Q9H6Q1, Q9H828, Q9UF52, B4DRL1 D3DPK4, F8W7K4, |
| UniProt Related Accession: | Q6P2H3 |
| Molecular Weight: | 80,302 Da |
| NCBI Full Name: | centrosomal protein of 85 kDa isoform 2 |
| NCBI Synonym Full Names: | centrosomal protein 85kDa |
| NCBI Official Symbol: | CEP85 |
| NCBI Official Synonym Symbols: | CCDC21 |
| NCBI Protein Information: | centrosomal protein of 85 kDa |
| UniProt Protein Name: | Centrosomal protein of 85 kDa |
| UniProt Synonym Protein Names: | Coiled-coil domain-containing protein 21 |
| Protein Family: | Centrosomal protein |
| UniProt Gene Name: | CEP85 |
| UniProt Entry Name: | CEP85_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CEP85 antibody (CAB15891) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of C6 cells using CEP85 antibody (CAB15891) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells using CEP85 antibody (CAB15891) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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