Cell Biology Antibodies 11

Anti-CENPK Antibody (CAB7627)

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SKU:
CAB7627
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CENPK Antibody
Antibody SKU:CAB7627
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-269 of human CENPK (NP_071428.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human
Positive Samples:HeLa, SKOV3, 293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-269 of human CENPK (NP_071428.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MNQE DLDP DSTT DVGD VTNT EEEL IREC EEMW KDME ECQN KLSL IGTE TLTD SNAQ LSLL IMQV KCLT AELS QWQK KTPE TIPL TEDV LITL GKEE FQKL RQDL EMVL STKE SKNE KLKE DLER EQRW LDEQ QQIM ESLN VLHS ELKN KVET FSES RIFN ELKT KMLN IKEY KEKL LSTL GEFL EDHF PLPD RSVK KKKK NIQE SSVN LITL HEML EILI NRLF DVPH DPYV KISD SFWP PYVE LLLR NGIA LRHP EDPT RIRL EAFH Q
Gene ID:64105
Uniprot:Q9BS16
Cellular Location:Chromosome, Nucleus, centromere, kinetochore
Calculated MW:31kDa
Observed MW:37kDa
Synonyms:CENPK, AF5alpha, CENP-K, FKSG14, P33, Solt
Background:
UniProt Protein Function:CENPK: Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with CASC5/KNL1 to recruit the NDC80 complex to the outer kinetochore. Chromosomal aberrations involving CENPK are a cause of acute leukemias. Translocation t(5;11)(q12;q23) with MLL. Belongs to the CENPK family.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 5q12.3

Cellular Component: cytosol; nucleoplasm

Molecular Function:protein binding

Biological Process: DNA replication-independent nucleosome assembly at centromere; sister chromatid cohesion

NCBI Summary:CENPK is a subunit of a CENPH (MIM 605607)-CENPI (MIM 300065)-associated centromeric complex that targets CENPA (MIM 117139) to centromeres and is required for proper kinetochore function and mitotic progression (Okada et al., 2006 [PubMed 16622420]).[supplied by OMIM, Mar 2008]
UniProt Code:Q9BS16
NCBI GenInfo Identifier:74732954
NCBI Gene ID:64105
NCBI Accession:Q9BS16.1
UniProt Secondary Accession:Q9BS16,Q9H4L0,
UniProt Related Accession:Q9BS16
Molecular Weight:31,655 Da
NCBI Full Name:Centromere protein K
NCBI Synonym Full Names:centromere protein K
NCBI Official Symbol:CENPK  
NCBI Official Synonym Symbols:P33; Solt; CENP-K; FKSG14; AF5alpha  
NCBI Protein Information:centromere protein K
UniProt Protein Name:Centromere protein K
UniProt Synonym Protein Names:Interphase centromere complex protein 37; Protein AF-5alpha; p33
Protein Family:Centromere protein
UniProt Gene Name:CENPK  
UniProt Entry Name:CENPK_HUMAN
Anti-CENPK Antibody (CAB7627)Western blot analysis of extracts of various cell lines, using CENPK Antibody (CAB7627) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-CENPK Antibody (CAB7627)Immunofluorescence analysis of U2OS cells using CENPK antibody (CAB7627) at dilution of 1:100. Blue: DAPI for nuclear staining.
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