Cell Biology Antibodies 12

Anti-CENPJ Antibody (CAB8721)

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SKU:
CAB8721
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CENPJ Antibody
Antibody SKU:CAB8721
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1069-1338 of human CENPJ (NP_060921.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:Raji
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1069-1338 of human CENPJ (NP_060921.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:DKLA NTSV RFQN SQIS SGTQ VEKY KKNY LPMQ GNPP RRSK SAPP RDLG NLDK GQAA SPRE PLEP LNFP DPEY KEEE EDQD IQGE ISHP DGKV EKVY KNGC RVIL FPNG TRKE VSAD GKTI TVTF FNGD VKQV MPDQ RVIY YYAA AQTT HTTY PEGL EVLH FSSG QIEK HYPD GRKE ITFP DQTV KNLF PDGQ EESI FPDG TIVR VQRD GNKL IEFN NGQR ELHT AQFK RREY PDGT VKTV YANG HQET KYRS GRIR VKDK EGNV LMDT EL
Gene ID:55835
Uniprot:Q9HC77
Cellular Location:Cytoplasm, centriole, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:124kDa/153kDa
Observed MW:153kDa
Synonyms:CENPJ, BM032, CENP-J, CPAP, LAP, LIP1, MCPH6, SASS4, SCKL4, Sas-4
Background:This gene encodes a protein that belongs to the centromere protein family. During cell division, this protein plays a structural role in the maintenance of centrosome integrity and normal spindle morphology, and it is involved in microtubule disassembly at the centrosome. This protein can function as a transcriptional coactivator in the Stat5 signaling pathway, and also as a coactivator of NF-kappaB-mediated transcription, likely via its interaction with the coactivator p300/CREB-binding protein. Mutations in this gene are associated with primary autosomal recessive microcephaly, a disorder characterized by severely reduced brain size and mental retardation. Alternatively spliced transcript variants have been found for this gene.
UniProt Protein Function:CENPJ: Plays an important role in cell division and centrosome function by participating in centriole duplication. Inhibits microtubule nucleation from the centrosome. Defects in CENPJ are the cause of microcephaly primary type 6 (MCPH6). A disorder defined as a head circumference more than 3 standard deviations below the age- related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits. Defects in CENPJ are the cause of Seckel syndrome type 4 (SCKL4). SCKL4 is a rare autosomal recessive disorder characterized by proportionate dwarfism of prenatal onset associated with low birth weight, growth retardation, severe microcephaly with a bird-headed like appearance, and mental retardation. Belongs to the TCP10 family.
UniProt Protein Details:

Protein type:Cell cycle regulation; Microtubule-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 13q12.12-q12.13

Cellular Component: centriole; centrosome; cytosol; gamma-tubulin small complex; microtubule; nucleoplasm; plasma membrane

Molecular Function:protein binding; protein domain specific binding; protein kinase binding; tubulin binding

Biological Process: cell division; centriole replication; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; G2/M transition of mitotic cell cycle; microtubule nucleation; microtubule polymerization; regulation of centriole replication; regulation of G2/M transition of mitotic cell cycle

Disease: Microcephaly 6, Primary, Autosomal Recessive; Seckel Syndrome 4

NCBI Summary:This gene encodes a protein that belongs to the centromere protein family. During cell division, this protein plays a structural role in the maintenance of centrosome integrity and normal spindle morphology, and it is involved in microtubule disassembly at the centrosome. This protein can function as a transcriptional coactivator in the Stat5 signaling pathway, and also as a coactivator of NF-kappaB-mediated transcription, likely via its interaction with the coactivator p300/CREB-binding protein. Mutations in this gene are associated with primary autosomal recessive microcephaly, a disorder characterized by severely reduced brain size and cognitive disability. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2012]
UniProt Code:Q9HC77
NCBI GenInfo Identifier:130980075
NCBI Gene ID:55835
NCBI Accession:NP_060921.3
UniProt Secondary Accession:Q9HC77,Q2KHM6, Q5JPD5, Q5T6R5, Q96KS5, Q9C067,
UniProt Related Accession:Q9HC77
Molecular Weight:124,244 Da
NCBI Full Name:centromere protein J
NCBI Synonym Full Names:centromere protein J
NCBI Official Symbol:CENPJ  
NCBI Official Synonym Symbols:LAP; CPAP; LIP1; BM032; MCPH6; SASS4; SCKL4; Sas-4; CENP-J  
NCBI Protein Information:centromere protein J
UniProt Protein Name:Centromere protein J
UniProt Synonym Protein Names:Centrosomal P4.1-associated protein; LAG-3-associated protein; LYST-interacting protein 1
Protein Family:Centromere protein
UniProt Gene Name:CENPJ  
Anti-CENPJ Polyclonal Antibody (CAB8721)Western blot analysis of extracts of Raji cells, using CENPJ Antibody (CAB8721) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
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