Cell Biology Antibodies 12

Anti-CENPH Antibody (CAB8372)

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SKU:
CAB8372
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CENPH Antibody
Antibody SKU:CAB8372
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-247 of human CENPH (NP_075060.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HeLa, NIH/3T3, 293T, OVCAR3, A673
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-247 of human CENPH (NP_075060.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MEEQ PQMQ DADE PADS GGEG RAGG PPQV AGAQ AACS EDRM TLLL RLRA QTKQ QLLE YKSM VDAS EEKT PEQI MQEK QIEA KIED LENE IEEV KVAF EIKK LALD RMRL STAL KKNL EKIS RQSS VLMD NMKH LLEL NKLI MKSQ QESW DLEE KLLD IRKK RLQL KQAS ESKL LEIQ TEKN KQKI DLDS MENS ERIK IIRQ NLQM EIKI TTVI QHVF QNLI LGSK VNWA EDPA LKEI VLQL EKNV DMM
Gene ID:64946
Uniprot:Q9H3R5
Cellular Location:Chromosome, Nucleus, centromere, kinetochore
Calculated MW:28kDa
Observed MW:38kDa
Synonyms:CENPH
Background:Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex.
UniProt Protein Function:CENPH: Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate. Belongs to the centromere protein H family.
UniProt Protein Details:

Protein type:Cell cycle regulation

Chromosomal Location of Human Ortholog: 5p15.2

Cellular Component: cytosol; kinetochore; nucleoplasm; nucleus

Molecular Function:kinetochore binding; protein binding

Biological Process: DNA replication-independent nucleosome assembly at centromere; kinetochore organization and biogenesis; sister chromatid cohesion

NCBI Summary:Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex. [provided by RefSeq, Jul 2008]
UniProt Code:Q9H3R5
NCBI GenInfo Identifier:74733576
NCBI Gene ID:64946
NCBI Accession:Q9H3R5.1
UniProt Secondary Accession:Q9H3R5,A8K3Y1,
UniProt Related Accession:Q9H3R5
Molecular Weight:28,481 Da
NCBI Full Name:Centromere protein H
NCBI Synonym Full Names:centromere protein H
NCBI Official Symbol:CENPH  
NCBI Protein Information:centromere protein H
UniProt Protein Name:Centromere protein H
UniProt Synonym Protein Names:Interphase centromere complex protein 35
UniProt Gene Name:CENPH  
UniProt Entry Name:CENPH_HUMAN
Anti-CENPH Polyclonal Antibody (CAB8372)Western blot analysis of extracts of various cell lines, using CENPH antibody (CAB8372) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
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