Epigenetics & Nuclear Signaling Antibodies 2

Anti-CELF2 Antibody (CAB6016)

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SKU:
CAB6016
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-CELF2 Antibody
Antibody SKU:CAB6016
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 422-521 of human CELF2 (NP_006552.3).
Application:WB
Recommended Dilution:WB 1:200 - 1:500
Reactivity:Human
Positive Samples:MCF7, HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 422-521 of human CELF2 (NP_006552.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:QQSA AGSQ KEGP EGAN LFIY HLPQ EFGD QDIL QMFM PFGN VISA KVFI DKQT NLSK CFGF VSYD NPVS AQAA IQAM NGFQ IGMK RLKV QLKR SKND SKPY
Gene ID:10659
Uniprot:O95319
Cellular Location:Cytoplasm, Nucleus
Calculated MW:52kDa/54kDa/55kDa
Observed MW:60kDa
Synonyms:CELF2, BRUNOL3, CELF-2, CUG-BP2, CUGBP2, ETR-3, ETR3, NAPOR
Background:Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Alternative splicing results in multiple transcript variants encoding different isoforms.
UniProt Protein Function:CELF2: RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of TNNT2 in embryonic, but not adult, skeletal muscle. Activates TNNT2 exon 5 inclusion by antagonizing the repressive effect of PTB. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Promotes inclusion of exonS 21 and exclusion of exon 5 of the NMDA receptor R1 pre- mRNA. Involved in the apoB RNA editing activity. Increases COX2 mRNA stability and inhibits COX2 mRNA translation in epithelial cells after radiation injury. Modulates the cellular apoptosis program by regulating COX2-mediated prostaglandin E2 (PGE2) expression. Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK. Binds to the muscle-specific splicing enhancer (MSE) intronic sites flanking the TNNT2 alternative exon 5. Binds preferentially to UG-rich sequences, in particular UG repeat and UGUU motifs. Binds to apoB mRNA, specifically to AU-rich sequences located immediatly upstream of the edited cytidine. Binds AU-rich sequences in the 3'-UTR of COX2 mRNA. Binds to an intronic RNA element responsible for the silencing of exon 21 splicing. Binds to (CUG)n repeats. Belongs to the CELF/BRUNOL family. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:RNA-binding

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: cytoplasm; nucleus

Molecular Function:RNA binding; nucleotide binding

Biological Process: RNA processing; mRNA processing; regulation of heart contraction

NCBI Summary:Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:O95319
NCBI GenInfo Identifier:74754421
NCBI Gene ID:10659
NCBI Accession:O95319.1
UniProt Secondary Accession:O95319,Q7KYU4, Q8N499, Q92950, Q96NW9, Q96RQ5, Q96RQ6 Q9UL67, B7ZAN9,
UniProt Related Accession:O95319
Molecular Weight:508
NCBI Full Name:CUGBP Elav-like family member 2
NCBI Synonym Full Names:CUGBP, Elav-like family member 2
NCBI Official Symbol:CELF2  
NCBI Official Synonym Symbols:ETR3; ETR-3; NAPOR; CUGBP2; BRUNOL3  
NCBI Protein Information:CUGBP Elav-like family member 2; CELF-2; CUG-BP2; bruno-like protein 3; RNA-binding protein BRUNOL-3; CUG-BP- and ETR-3-like factor 2; ELAV-type RNA-binding protein 3; CUG triplet repeat RNA-binding protein 2; neuroblastoma apoptosis-related RNA-binding protein
UniProt Protein Name:CUGBP Elav-like family member 2
UniProt Synonym Protein Names:Bruno-like protein 3; CUG triplet repeat RNA-binding protein 2; CUG-BP2; CUG-BP- and ETR-3-like factor 2; ELAV-type RNA-binding protein 3; ETR-3; Neuroblastoma apoptosis-related RNA-binding protein; hNAPOR; RNA-binding protein BRUNOL-3
Protein Family:CUGBP Elav-like family
UniProt Gene Name:CELF2  
UniProt Entry Name:CELF2_HUMAN
Anti-CELF2 Antibody (CAB6016)Western blot analysis of extracts of various cell lines, using CELF2 antibody (CAB6016) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.
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