Name: Anti-CDYL Antibody (CAB6258)
Brand: Developmental Biology
SKU: CAB6258
Availability: OutOfStock

Antibody Name:	Anti-CDYL Antibody
Antibody SKU:	CAB6258
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 245-544 of human CDYL (NP_004815.3).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Rat
Positive Samples:	U-251MG

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 245-544 of human CDYL (NP_004815.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TNIQ TSVT GVTA SKRK FIDD RRDQ PFDK RLRF SVRQ TESA YRYR DIVV RKQD GFTH ILLS TKSS ENNS LNPE VMRE VQSA LSTA AADD SKLV LLSA VGSV FCCG LDFI YFIR RLTD DRKR ESTK MAEA IRNF VNTF IQFK KPII VAVN GPAI GLGA SILP LCDV VWAN EKAW FQTP YTTF GQSP DGCS TVMF PKIM GGAS ANEM LLSG RKLT AQEA CGKG LVSQ VFWP GTFT QEVM VRIK ELAS CNPV VLEE SKAL VRCN MKME LEQA NERE CEVL KKIW GSAQ GMDS MLKY LQRK IDEF
Gene ID:	9425
Uniprot:	Q9Y232
Cellular Location:	Nucleus
Calculated MW:	34kDa/45kDa/60kDa/66kDa
Observed MW:	75kDa

Synonyms:

CDYL, CDYL1

Background:

Chromodomain Y is a primate-specific Y-chromosomal gene family expressed exclusively in the testis and implicated in infertility. Although the Y-linked genes are testis-specific, this autosomal gene is ubiquitously expressed. The Y-linked genes arose by retrotransposition of an mRNA from this gene, followed by amplification of the retroposed gene. Proteins encoded by this gene superfamily possess a chromodomain, a motif implicated in chromatin binding and gene suppression, and a catalytic domain believed to be involved in histone acetylation. Multiple proteins are encoded by transcript variants of this gene.

UniProt Protein Function:	CDYL: Acts as repressor of transcription. Has histone acetyltransferase activity, with a preference for histone H4. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Acetyltransferase; EC 2.3.1.48; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 6p25.1 Cellular Component: nucleoplasm; nucleus Molecular Function:protein binding; histone acetyltransferase activity; transcription corepressor activity; methylated histone residue binding Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; spermatogenesis; histone acetylation
NCBI Summary:	Chromodomain Y is a primate-specific Y-chromosomal gene family expressed exclusively in the testis and implicated in infertility. Although the Y-linked genes are testis-specific, this autosomal gene is ubiquitously expressed. The Y-linked genes arose by retrotransposition of an mRNA from this gene, followed by amplification of the retroposed gene. Proteins encoded by this gene superfamily possess a chromodomain, a motif implicated in chromatin binding and gene suppression, and a catalytic domain believed to be involved in histone acetylation. Multiple proteins are encoded by transcript variants of this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9Y232
NCBI GenInfo Identifier:	150421527
NCBI Gene ID:	9425
NCBI Accession:	Q9Y232.2
UniProt Secondary Accession:	Q9Y232,Q0VDG7, Q32NC5, Q5VX99, Q6P7T5, Q9BWZ2, Q9Y424 A8K6D6, B4DLG4,
UniProt Related Accession:	Q9Y232
Molecular Weight:	598
NCBI Full Name:	Chromodomain Y-like protein
NCBI Synonym Full Names:	chromodomain protein, Y-like
NCBI Official Symbol:	CDYL
NCBI Official Synonym Symbols:	CDYL1
NCBI Protein Information:	chromodomain Y-like protein; CDY-like, autosomal; testis-specific chromodomain Y-like protein
UniProt Protein Name:	Chromodomain Y-like protein
Protein Family:	Chromodomain Y-like protein
UniProt Gene Name:	CDYL
UniProt Entry Name:	CDYL1_HUMAN

Western blot analysis of extracts of U-251MG cells, using CDYL antibody (CAB6258) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

Antibodies

CDYL Antibody (PACO08357)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)