Cell Cycle Antibodies 1

CDKN1A/p21CIP1 Rabbit Polyclonal Antibody (CAB1483)

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SKU:
CAB1483
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle
Reactivity:
Human, Mouse, Rat
Synonyms:
P21, CIP1, SDI1, WAF1, CAP20, CDKN1, MDA-6, p21CIP1, CDKN1A/p21CIP1
Research Use:
Cell Cycle
Frequently bought together:

Description

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CDKN1A/p21CIP1 Antibody (CAB1483)

The CDKN1A/p21CIP1 Antibody (CAB1483) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it an ideal choice for experiments such as Western blotting.CDKN1A, also known as p21, is a crucial player in cell cycle control, functioning as a potent inhibitor of cyclin-dependent kinases. Dysregulation of this protein has been linked to various types of cancer, making it a promising target for cancer research.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:CDKN1A/p21CIP1 Antibody
SKU:CAB1483
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:MSEP AGDV RQNP CGSK ACRR LFGP VDSE QLSR DCDA LMAG CIQE ARER WNFD FVTE TPLE GDFA WERV RGLG LPKL YLPT GPRR GRDE LGGG RRPG TSPA LLQG TAEE DHVD LSLS CTLV PRSG EQAE GSPG GPGD SQGR KRRQ TSMT DFYH SKRR LIFS KRKP
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:P21, CIP1, SDI1, WAF1, CAP20, CDKN1, MDA-6, p21CIP1, CDKN1A/p21CIP1
Positive Sample:MCF7, Mouse large intestine, Mouse testis, Rat lung
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:18kDa
Observed MW:18kDa(Mouse)/21kDa(Human/Rat)

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene.

Purification MethodAffinity purification
Gene ID1026
RRIDAB_2761709
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from MCF7 cells, using CDKN1A/p21CIP1 Rabbit pAb (CAB1483) at 1:900 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Product ImageWestern blot analysis of various lysates, using CDKN1A/p21CIP1 Rabbit pAb (CAB1483) at 1:900 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Product ImageImmunofluorescence analysis of C6 cells using CDKN1A/p21CIP1 Rabbit pAb (CAB1483) at dilution of 1:100. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of HeLa cells using CDKN1A/p21CIP1 Rabbit pAb (CAB1483) at dilution of 1:100. Blue: DAPI for nuclear staining.
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