Epigenetics & Nuclear Signaling Antibodies 2
Anti-CDKAL1 Antibody (CAB5771)
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- SKU:
- CAB5771
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-CDKAL1 Antibody |
| Antibody SKU: | CAB5771 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human CDKAL1 (NP_060244.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:10 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HL-60, HeLa, BT-474, 293T, HepG2, Mouse kidney, Mouse heart, Mouse spleen, Mouse lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human CDKAL1 (NP_060244.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MPSA SCDT LLDD IEDI VSQE DSKP QDRH FVRK DVVP KVRR RNTQ KYLQ EEEN SPPS DSTI PGIQ KIWI RTWG CSHN NSDG EYMA GQLA AYGY KITE NASD ADLW LLNS CTVK NPAE DHFR NSIK KAQE ENKK IVLA GCVP QAQP RQDY LKGL SIIG VQQI DRVV EVVE ETIK GHSV RLLG QKKD NGRR LGGA RLDL PKIR KNPL IEII SINT GCLN ACTY CKTK HARG NL |
| Gene ID: | 54901 |
| Uniprot: | Q5VV42 |
| Cellular Location: | Endoplasmic reticulum membrane, Single-pass membrane protein |
| Calculated MW: | 11kDa/54kDa/65kDa |
| Observed MW: | 55-65kDa |
| Synonyms: | CDKAL1 |
| Background: | The protein encoded by this gene is a member of the methylthiotransferase family. The function of this gene is not known. Genome-wide association studies have linked single nucleotide polymorphisms in an intron of this gene with susceptibilty to type 2 diabetes. |
| UniProt Protein Function: | CDKAL1: Catalyzes the methylthiolation of N6- threonylcarbamoyladenosine (t(6)A), leading to the formation of 2- methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine. Belongs to the methylthiotransferase family. CDKAL1 subfamily. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; EC 2.8.4.5; Enzyme, misc. Chromosomal Location of Human Ortholog: 6p22.3 Cellular Component: endoplasmic reticulum membrane; rough endoplasmic reticulum; membrane; integral to membrane Molecular Function:4 iron, 4 sulfur cluster binding; metal ion binding Disease: Diabetes Mellitus, Noninsulin-dependent |
| NCBI Summary: | The protein encoded by this gene is a member of the methylthiotransferase family. The function of this gene is not known. Genome-wide association studies have linked single nucleotide polymorphisms in an intron of this gene with susceptibilty to type 2 diabetes. [provided by RefSeq, May 2010] |
| UniProt Code: | Q5VV42 |
| NCBI GenInfo Identifier: | 74747199 |
| NCBI Gene ID: | 54901 |
| NCBI Accession: | Q5VV42.1 |
| UniProt Secondary Accession: | Q5VV42,Q6P385, Q6ZR27, Q9NXB3, A8K6S0, |
| UniProt Related Accession: | Q5VV42 |
| Molecular Weight: | 579 |
| NCBI Full Name: | Threonylcarbamoyladenosine tRNA methylthiotransferase |
| NCBI Synonym Full Names: | CDK5 regulatory subunit associated protein 1-like 1 |
| NCBI Official Symbol: | CDKAL1 |
| NCBI Protein Information: | threonylcarbamoyladenosine tRNA methylthiotransferase; tRNA-t(6)A37 methylthiotransferase |
| UniProt Protein Name: | Threonylcarbamoyladenosine tRNA methylthiotransferase |
| UniProt Synonym Protein Names: | CDK5 regulatory subunit-associated protein 1-like 1; tRNA-t(6)A37 methylthiotransferase |
| Protein Family: | Threonylcarbamoyladenosine tRNA methylthiotransferase |
| UniProt Gene Name: | CDKAL1 |
| UniProt Entry Name: | CDKAL_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CDKAL1 antibody (CAB5771) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of U2OS cells using CDKAL1 antibody (CAB5771). Blue: DAPI for nuclear staining. |
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