Antibody Name:	Anti-CDK9 Antibody
Antibody SKU:	CAB1564
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 153-372 of human CDK9 (NP_001252.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:1000 - 1:3000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	A549, HeLa, U-251MG, Raji, BT-474, Mouse spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 153-372 of human CDK9 (NP_001252.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ANVL ITRD GVLK LADF GLAR AFSL AKNS QPNR YTNR VVTL WYRP PELL LGER DYGP PIDL WGAG CIMA EMWT RSPI MQGN TEQH QLAL ISQL CGSI TPEV WPNV DNYE LYEK LELV KGQK RKVK DRLK AYVR DPYA LDLI DKLL VLDP AQRI DSDD ALNH DFFW SDPM PSDL KGML STHL TSMF EYLA PPRR KGSQ ITQQ STNQ SRNP ATTN QTEF ERVF
Gene ID:	1025
Uniprot:	P50750
Cellular Location:	Cytoplasm, Nucleus, PML body
Calculated MW:	42kDa/53kDa
Observed MW:	40kDa

Synonyms:

CDK9, C-2k, CDC2L4, CTK1, PITALRE, TAK

Background:

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.

UniProt Protein Function:	CDK9: a protein kinase of the CDK family. Forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. A component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. Transcriptional elongation factor and cofactor for HIV Tat protein; RNAi blocks HIV replication, and inhibitors also block varicella zoster replication. Mediates signals leading to cardiac hypertrophy. Inhibitor: Flavopiridol.
UniProt Protein Details:	Protein type:Kinase, protein; Cell cycle regulation; Nuclear receptor co-regulator; EC 2.7.11.22; EC 2.7.11.23; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); CMGC group; CDK family; CDK9 subfamily; CDK/CDK9 subfamily Chromosomal Location of Human Ortholog: 9q34.1 Cellular Component: nucleoplasm; transcription elongation factor complex; PML body; transcription elongation factor complex b; intracellular membrane-bound organelle; membrane; cytoplasm Molecular Function:RNA polymerase subunit kinase activity; protein binding; snRNA binding; DNA binding; cyclin-dependent protein kinase activity; chromatin binding; ATP binding; protein kinase activity Biological Process: regulation of histone modification; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; positive regulation of histone phosphorylation; transcription, DNA-dependent; viral reproduction; positive regulation of viral transcription; regulation of mitotic cell cycle; DNA repair; protein amino acid phosphorylation; regulation of DNA repair; cell proliferation; transforming growth factor beta receptor signaling pathway; RNA elongation from RNA polymerase II promoter; replication fork arrest; gene expression; positive regulation of transcription from RNA polymerase II promoter
NCBI Summary:	The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. [provided by RefSeq, Jul 2008]
UniProt Code:	P50750
NCBI GenInfo Identifier:	68067660
NCBI Gene ID:	1025
NCBI Accession:	P50750.3
UniProt Secondary Accession:	P50750,Q5JU24, Q5JU25, Q5U006, Q96TF1,
UniProt Related Accession:	P50750
Molecular Weight:	372
NCBI Full Name:	Cyclin-dependent kinase 9
NCBI Synonym Full Names:	cyclin-dependent kinase 9
NCBI Official Symbol:	CDK9
NCBI Official Synonym Symbols:	TAK; C-2k; CTK1; CDC2L4; PITALRE
NCBI Protein Information:	cyclin-dependent kinase 9; CDC2-related kinase; cell division protein kinase 9; serine/threonine protein kinase PITALRE; cell division cycle 2-like protein kinase 4; tat-associated kinase complex catalytic subunit
UniProt Protein Name:	Cyclin-dependent kinase 9
UniProt Synonym Protein Names:	C-2K; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; Serine/threonine-protein kinase PITALRE; Tat-associated kinase complex catalytic subunit
Protein Family:	Cyclin-dependent kinase
UniProt Gene Name:	CDK9
UniProt Entry Name:	CDK9_HUMAN

	Western blot analysis of extracts of various cell lines, using CDK9 antibody (CAB1564) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of U2OS cells using CDK9 antibody (CAB1564). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug CDK9 antibody (CAB1564). Western blot was performed from the immunoprecipitate using CDK9 antibody (CAB1564) at a dilition of 1:1000.