Epigenetics & Nuclear Signaling Antibodies 5
Anti-CDK9 Antibody (CAB11145)
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- SKU:
- CAB11145
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Monoclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-CDK9 Antibody |
| Antibody SKU: | CAB11145 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthesized peptide derived from human CDK9 |
| Application: | WB IHC IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, 293T, MCF7, Mouse brain, Rat testis, Rat brain |
| Immunogen: | A synthesized peptide derived from human CDK9 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 1025 |
| Uniprot: | P50750 |
| Cellular Location: | Cytoplasm, Nucleus, PML body |
| Calculated MW: | 43kDa |
| Observed MW: | 42KDa/55KDa |
 | Western blot analysis of extracts of various cell lines, using CDK9 Rabbit mAb at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min. |
 | Immunohistochemistry of paraffin-embedded rat ovary using CDK9 Rabbit mAb at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded human esophageal using CDK9 Rabbit mAb at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded mouse brain using CDK9 Rabbit mAb at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol. |
 | Immunofluorescence analysis of HeLa cells using CDK9 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH-3T3 cells using CDK9 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug CDK9 antibody at a dilition of 1:1000. |
 | Chromatin immunoprecipitations were performed with cross-linked chromatin from DLD-1 cells and CDK9 Rabbit mAb (A11145). The ChIP sequencing results indicate the enrichment pattern of CDK9 in selected genomic region and representative gene loci (e. g. RAC1, GAPDH and ACTB), as shown in figure. |
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