Antibody Name:	CDK16 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8140
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 277-496 of human CDK16 (NP_006192.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	BT-474, Mouse pancreas

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 277-496 of human CDK16 (NP_006192.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	CHRQ KVLH RDLK PQNL LINE RGEL KLAD FGLA RAKS IPTK TYSN EVVT LWYR PPDI LLGS TDYS TQID MWGV GCIF YEMA TGRP LFPG STVE EQLH FIFR ILGT PTEE TWPG ILSN EEFK TYNY PKYR AEAL LSHA PRLD SDGA DLLT KLLQ FEGR NRIS AEDA MKHP FFLS LGER IHKL PDTT SIFA LKEI QLQK EASL RSSS MPDS GRPA FRVV DTEF
Gene ID:	5127
Uniprot:	Q00536
Cellular Location:	Cell junction, Cell membrane, Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Peripheral membrane protein, secretory vesicle, synapse, synaptosome
Calculated MW:	55kDa/56kDa/63kDa
Observed MW:	56kDa

Synonyms:

CDK16, PCTAIRE, PCTAIRE1, PCTGAIRE, PCTK1

Background:

The protein encoded by this gene belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. It may play a role in signal transduction cascades in terminally differentiated cells; in exocytosis; and in transport of secretory cargo from the endoplasmic reticulum. This gene is thought to escape X inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	CDK16: May play a role in signal transduction cascades in terminally differentiated cells. Found in a complex containing CABLES1, CDK17 and TDRD7. Interacts with YWHAH, YWHAQ and YWHAZ. Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
UniProt Protein Details:	Protein type:Protein kinase, Ser/Thr (non-receptor); Motility/polarity/chemotaxis; Cell cycle regulation; EC 2.7.11.22; Protein kinase, CMGC; Kinase, protein; CMGC group; CDK family; TAIRE subfamily; CDK/TAIRE subfamily Chromosomal Location of Human Ortholog: Xp11 Cellular Component: microtubule cytoskeleton; synaptic vesicle; extrinsic to internal side of plasma membrane; neuron projection; transport vesicle; cytoplasm; plasma membrane; cell junction Molecular Function:protein serine/threonine kinase activity; protein binding; cyclin-dependent protein kinase activity; ATP binding Biological Process: growth hormone secretion; exocytosis; regulation of cell cycle; spermatogenesis; protein amino acid phosphorylation; neurite development
NCBI Summary:	The protein encoded by this gene belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. It may play a role in signal transduction cascades in terminally differentiated cells; in exocytosis; and in transport of secretory cargo from the endoplasmic reticulum. This gene is thought to escape X inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2009]
UniProt Code:	Q00536
NCBI GenInfo Identifier:	266425
NCBI Gene ID:	5127
NCBI Accession:	Q00536.1
UniProt Secondary Accession:	Q00536,A8K280, B7Z7C8, J3KN74, J3KQP7,
UniProt Related Accession:	Q00536
Molecular Weight:	56,375 Da
NCBI Full Name:	Cyclin-dependent kinase 16
NCBI Synonym Full Names:	cyclin-dependent kinase 16
NCBI Official Symbol:	CDK16
NCBI Official Synonym Symbols:	PCTK1; PCTAIRE; PCTAIRE1; PCTGAIRE
NCBI Protein Information:	cyclin-dependent kinase 16; PCTAIRE-motif protein kinase 1; cell division protein kinase 16; serine/threonine-protein kinase PCTAIRE-1
UniProt Protein Name:	Cyclin-dependent kinase 16
UniProt Synonym Protein Names:	Cell division protein kinase 16; PCTAIRE-motif protein kinase 1; Serine/threonine-protein kinase PCTAIRE-1
UniProt Gene Name:	CDK16
UniProt Entry Name:	CDK16_HUMAN

	Western blot analysis of extracts of various cell lines, using CDK16 antibody (CAB8140) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of U2OS cells using CDK16 antibody (CAB8140) at dilution of 1:100. Blue: DAPI for nuclear staining.