Cell Cycle Antibodies 1

Anti-CDC7 Antibody (CAB5738)

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SKU:
CAB5738
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-CDC7 Antibody
Antibody SKU:CAB5738
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 275-574 of human CDC7 (NP_001127892.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:SH-SY5Y, HepG2, THP-1
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 275-574 of human CDC7 (NP_001127892.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EGSV GLSV QRSV FGER NFNI HSSI SHES PAVK LMKQ SKTV DVLS RKLA TKKK AIST KVMN SAVM RKTA SSCP ASLT CDCY ATDK VCSI CLSR RQQV APRA GTPG FRAP EVLT KCPN QTTA IDMW SAGV IFLS LLSG RYPF YKAS DDLT ALAQ IMTI RGSR ETIQ AAKT FGKS ILCS KEVP AQDL RKLC ERLR GMDS STPK LTSD IQGH ASHQ PAIS EKTD HKAS CLVQ TPPG QYSG NSFK KGDS NSCE HCFD EYNT NLEG WNEV PDEA YDLL DKLL DLNP ASRI TAEE ALLH PFFK DMSL
Gene ID:8317
Uniprot:O00311
Cellular Location:Nucleus
Calculated MW:63kDa
Observed MW:70kDa
Synonyms:CDC7, CDC7L1, HsCDC7, Hsk1, huCDC7
Background:This gene encodes a cell division cycle protein with kinase activity that is critical for the G1/S transition. The yeast homolog is also essential for initiation of DNA replication as cell division occurs. Overexpression of this gene product may be associated with neoplastic transformation for some tumors. Multiple alternatively spliced transcript variants that encode the same protein have been detected.
UniProt Protein Function:CDC7: an evolutionarily conserved serine/threonine protein kinase of the CDC7 family. Predominantly localized in the nucleus, it plays a pivotal role in linking cell cycle regulation to genome duplication, being essential for the firing of DNA replication origins. Plays an essential role in initiating DNA replication at replication origins by phosphorylating the MCM2 and MCM4 subunits of the MCM2-7 helicase complex. The human protein requires phosphorylation by CDK1 at T376 and binding to activator of S-phase kinase (ASK)/DBF4B to be fully activated. The interaction between Cdc7 and DBFK subunits facilitates ATP binding and substrate recognition by the Cdc7 kinase. Although CDC7 expression levels appear to be constant throughout the cell cycle, the expression levels of DBF4B is cell cycle-regulated. Thus, the CDC7 activity is activated during S phase in eukaryotes. The Cdc7/ DBF4B complex regulates initiation of DNA replication by phosphorylating chromatin-bound mini-chromosome maintenance protein 2 (MCM2). Impaired Cdc7 kinase activity leads to S-phase arrest. Plays a role in the TGF-beta regulation of smooth muscle cell (SMC) differentiation. Interacts with Smad3 to facilitate Smad3 binding to SMC marker promoter, leading to the activation of SMC marker gene transcription.
UniProt Protein Details:

Protein type:Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; EC 2.7.11.1; Other group; CDC7 family

Chromosomal Location of Human Ortholog: 1p22

Cellular Component: nucleoplasm; microtubule cytoskeleton; intercellular bridge; cytoplasm; nucleus

Molecular Function:protein serine/threonine kinase activity; protein binding; metal ion binding; kinase activity; ATP binding; protein kinase activity

Biological Process: regulation of cell shape; peptidyl-serine phosphorylation; DNA replication initiation; cell division; positive regulation of cell proliferation; mitotic cell cycle; DNA replication; phagocytosis; double-strand break repair via break-induced replication; G1/S transition of mitotic cell cycle

NCBI Summary:This gene encodes a cell division cycle protein with kinase activity that is critical for the G1/S transition. The yeast homolog is also essential for initiation of DNA replication as cell division occurs. Overexpression of this gene product may be associated with neoplastic transformation for some tumors. Multiple alternatively spliced transcript variants that encode the same protein have been detected. [provided by RefSeq, Aug 2008]
UniProt Code:O00311
NCBI GenInfo Identifier:12643528
NCBI Gene ID:8317
NCBI Accession:O00311.1
UniProt Secondary Accession:O00311,O00558, Q5T5U5, D3DT31,
UniProt Related Accession:O00311
Molecular Weight:574
NCBI Full Name:Cell division cycle 7-related protein kinase
NCBI Synonym Full Names:cell division cycle 7
NCBI Official Symbol:CDC7  
NCBI Official Synonym Symbols:Hsk1; CDC7L1; HsCDC7; huCDC7  
NCBI Protein Information:cell division cycle 7-related protein kinase; CDC7-related kinase; cell division cycle 7 homolog; cell division cycle 7-like protein 1; CDC7 (cell division cycle 7, S. cerevisiae, homolog)-like 1
UniProt Protein Name:Cell division cycle 7-related protein kinase
Protein Family:Cell division control protein
UniProt Gene Name:CDC7  
UniProt Entry Name:CDC7_HUMAN
Anti-CDC7 Antibody (CAB5738)Western blot analysis of extracts of various cell lines, using CDC7 antibody (CAB5738) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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