Cell Cycle Antibodies 1

Anti-CDC5L Antibody (CAB5560)

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SKU:
CAB5560
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-CDC5L Antibody
Antibody SKU:CAB5560
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 563-802 of human CDC5L (NP_001244.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:Human, Mouse
Positive Samples:MCF7, THP-1, K-562, SW480, Mouse spleen, Mouse thymus, Mouse testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 563-802 of human CDC5L (NP_001244.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PPLT DLQK SEEL IKKE MITM LHYD LLHH PYEP SGNK KGKT VGFG TNNS EHIT YLEH NPYE KFSK EELK KAQD VLVQ EMEV VKQG MSHG ELSS EAYN QVWE ECYS QVLY LPGQ SRYT RANL ASKK DRIE SLEK RLEI NRGH MTTE AKRA AKME KKMK ILLG GYQS RAMG LMKQ LNDL WDQI EQAH LELR TFEE LKKH EDSA IPRR LECL KEDV QRQQ EREK ELQH RYAD LLLE KETL KSKF
Gene ID:988
Uniprot:Q99459
Cellular Location:Cytoplasm, Nucleus, Nucleus speckle
Calculated MW:92kDa
Observed MW:107kDa
Synonyms:CDC5L, CDC5, CDC5-LIKE, CEF1, PCDC5RP, dJ319D22.1
Background:The protein encoded by this gene shares a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G2/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G2/M progression. It was also found to be an essential component of a non-snRNA spliceosome, which contains at least five additional protein factors and is required for the second catalytic step of pre-mRNA splicing.
UniProt Protein Function:CDC5L: is a spliceosome protein that binds DNA and that also regulates transcription. Is a positive regulator of cell cycle G2/M progression. Is an essential component of non-snRNA spliceosomes and is required for the second catalytic step of pre-mRNA splicing. Binds adeno-pre-mRNA in an ATP-stimulated manner. Is part of a spliceosomal 'core' complex that includes CDC5L, SF2, PRLI, SPF27, HSP7C, PRPF19/SNEV, GCN1L. Identified in the spliceosome C complex. Has been associated with aneuploidy in aging cells and in tumorigenesis. The genomic region containing the gene for CDC5L (6p21.1) is frequently amplified in osteosarcomas.
UniProt Protein Details:

Protein type:DNA-binding; RNA processing; Transcription factor; Spliceosome; Motility/polarity/chemotaxis; RNA splicing

Chromosomal Location of Human Ortholog: 6p21

Cellular Component: nucleoplasm; DNA replication factor A complex; membrane; cytoplasm; nucleolus; nuclear speck; nucleus

Molecular Function:protein binding; DNA binding; chromatin binding

Biological Process: nuclear mRNA splicing, via spliceosome; transcription, DNA-dependent; regulation of transcription, DNA-dependent; RNA splicing; gene expression; DNA repair

NCBI Summary:The protein encoded by this gene shares a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G2/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G2/M progression. It was also found to be an essential component of a non-snRNA spliceosome, which contains at least five additional protein factors and is required for the second catalytic step of pre-mRNA splicing. [provided by RefSeq, Jul 2008]
UniProt Code:Q99459
NCBI GenInfo Identifier:73619933
NCBI Gene ID:988
NCBI Accession:Q99459.2
UniProt Related Accession:Q99459
Molecular Weight:100kDa
NCBI Full Name:Cell division cycle 5-like protein
NCBI Synonym Full Names:cell division cycle 5 like
NCBI Official Symbol:CDC5L  
NCBI Official Synonym Symbols:CDC5; CEF1; PCDC5RP; CDC5-LIKE; dJ319D22.1  
NCBI Protein Information:cell division cycle 5-like protein
UniProt Protein Name:Cell division cycle 5-like protein
UniProt Synonym Protein Names:Pombe cdc5-related protein
Protein Family:CDC50 family protein
UniProt Gene Name:CDC5L  
UniProt Entry Name:CDC5L_HUMAN
Anti-CDC5L Antibody (CAB5560)Western blot analysis of extracts of various cell lines, using CDC5L antibody (CAB5560) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-CDC5L Antibody (CAB5560)Immunofluorescence analysis of A549 cells using CDC5L antibody (CAB5560).
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