Cell Cycle Antibodies 1

Anti-CDC45 Antibody (CAB16032)

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SKU:
CAB16032
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-CDC45 Antibody
Antibody SKU:CAB16032
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant protein of human CDC45
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human
Positive Samples:
Immunogen:Recombinant protein of human CDC45
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:8318
Uniprot:O75419
Cellular Location:
Calculated MW:60kDa/65kDa/68kDa
Observed MW:Refer to figures
Synonyms:CDC45, CDC45L, CDC45L2, MGORS7, PORC-PI-1, cdc45
Background:The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants.
UniProt Protein Function:CDC45L: Required for initiation of chromosomal DNA replication. Associated with ORC2. Widely expressed, highest levels are found in adult testis and thymus and in fetal liver. Belongs to the CDC45 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cell cycle regulation

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: nucleoplasm; centrosome; cytoplasm; nucleus

Molecular Function:protein binding; 3'-5' DNA helicase activity; DNA replication origin binding; chromatin binding; single-stranded DNA binding

Biological Process: G1/S-specific transcription in mitotic cell cycle; DNA replication initiation; regulation of chromatin silencing at telomere; mitotic cell cycle; DNA strand elongation during DNA replication; DNA replication checkpoint; DNA replication; DNA duplex unwinding; double-strand break repair via break-induced replication; G1/S transition of mitotic cell cycle; pre-replicative complex assembly

NCBI Summary:The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
UniProt Code:O75419
NCBI GenInfo Identifier:6685264
NCBI Gene ID:8318
NCBI Accession:O75419.1
UniProt Secondary Accession:O75419,O60856, Q20WK8, Q6UW54, Q9UP68, B4DDB4, B4DDU3 E9PDH7,
UniProt Related Accession:O75419
Molecular Weight:566
NCBI Full Name:Cell division control protein 45 homolog
NCBI Synonym Full Names:cell division cycle 45
NCBI Official Symbol:CDC45  
NCBI Official Synonym Symbols:CDC45L; CDC45L2; PORC-PI-1  
NCBI Protein Information:cell division control protein 45 homolog; human CDC45; CDC45-related protein; cell division cycle 45-like 2; cell division cycle 45 homolog; CDC45 cell division cycle 45 homolog
UniProt Protein Name:Cell division control protein 45 homolog
UniProt Synonym Protein Names:PORC-PI-1
UniProt Gene Name:CDC45  
UniProt Entry Name:CDC45_HUMAN
Anti-CDC45 Antibody (CAB16032)Immunofluorescence analysis of A-549 cells using cdc45 antibody (CAB16032). Blue: DAPI for nuclear staining.
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