Cell Cycle Antibodies 1

Anti-CDC23 Antibody (CAB15116)

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SKU:
CAB15116
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-CDC23 Antibody
Antibody SKU:CAB15116
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 50-150 of human CDC23 (NP_004652.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, 293T, HeLa, Jurkat, HepG2, NIH/3T3, mouse liver, mouse kidney, rat liver
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 50-150 of human CDC23 (NP_004652.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:HSSK WSAE LAFS LPAL PLAE LQPP PPIT EEDA QDMD AYTL AKAY FDVK EYDR AAHF LHGC NSKK AYFL YMYS RYLS GEKK KDDE TVDS LGPL EKGQ VKNE A
Gene ID:8697
Uniprot:Q9UJX2
Cellular Location:
Calculated MW:16kDa/55kDa/68kDa
Observed MW:69kDa
Synonyms:CDC23, ANAPC8, APC8, CUT23
Background:The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction.
UniProt Protein Function:CDC23: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Belongs to the APC8/CDC23 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cell cycle regulation

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: nucleoplasm; anaphase-promoting complex; intracellular; cytosol

Molecular Function:ubiquitin-protein ligase activity

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; ubiquitin-dependent protein catabolic process; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; regulation of mitotic metaphase/anaphase transition; mitotic cell cycle spindle assembly checkpoint; regulation of exit from mitosis; mitotic cell cycle; mitotic metaphase plate congression; mitotic metaphase/anaphase transition

NCBI Summary:The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. [provided by RefSeq, Jul 2008]
UniProt Code:Q9UJX2
NCBI GenInfo Identifier:254763423
NCBI Gene ID:8697
NCBI Accession:Q9UJX2.3
UniProt Secondary Accession:Q9UJX2,O75433, Q53FN2, Q9BS73, A8K6E5, B4E3A2, B7WP05 D3DQB7,
UniProt Related Accession:Q9UJX2
Molecular Weight:55,882 Da
NCBI Full Name:Cell division cycle protein 23 homolog
NCBI Synonym Full Names:cell division cycle 23
NCBI Official Symbol:CDC23  
NCBI Official Synonym Symbols:APC8; CUT23; ANAPC8  
NCBI Protein Information:cell division cycle protein 23 homolog; cyclosome subunit 8; cell division cycle 23 homolog; anaphase promoting complex subunit 8; anaphase-promoting complex subunit 8
UniProt Protein Name:Cell division cycle protein 23 homolog
UniProt Synonym Protein Names:Anaphase-promoting complex subunit 8; APC8; Cyclosome subunit 8
Protein Family:Anaphase-promoting complex
UniProt Gene Name:CDC23  
UniProt Entry Name:CDC23_HUMAN
Anti-CDC23 Antibody (CAB15116)Western blot analysis of extracts of various cell lines, using CDC23 antibody (CAB15116) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-CDC23 Antibody (CAB15116)Immunohistochemistry of paraffin-embedded rat spleen using CDC23 antibody (CAB15116) at dilution of 1:100 (40x lens).
Anti-CDC23 Antibody (CAB15116)Immunohistochemistry of paraffin-embedded human tonsil using CDC23 antibody (CAB15116) at dilution of 1:100 (40x lens).
Anti-CDC23 Antibody (CAB15116)Immunohistochemistry of paraffin-embedded mouse lung using CDC23 antibody (CAB15116) at dilution of 1:100 (40x lens).
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