Cell Cycle Antibodies 1
Anti-CDC20 Antibody (CAB1231)
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- SKU:
- CAB1231
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-CDC20 Antibody |
| Antibody SKU: | CAB1231 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human CDC20 (NP_001246.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | U-251MG, DU145, SW480, THP-1 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human CDC20 (NP_001246.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAQF AFES DLHS LLQL DAPI PNAP PARW QRKA KEAA GPAP SPMR AANR SHSA GRTP GRTP GKSS SKVQ TTPS KPGG DRYI PHRS AAQM EVAS FLLS KENQ PENS QTPT KKEH QKAW ALNL NGFD VEEA KILR LSGK PQNA PEGY QNRL KVLY SQKA TPGS SRKT CRYI PSLP DRIL DAPE IRND YYLN LVDW SSGN VLAV ALDN SVYL WSAS SGDI LQLL QMEQ PGEY ISSV AWIK EGNY LAVG TSSA EVQL WDVQ QQKR LRNM TSHS AR |
| Gene ID: | 991 |
| Uniprot: | Q12834 |
| Cellular Location: | Cytoplasm, centrosome, cytoskeleton, microtubule organizing center, spindle pole |
| Calculated MW: | 54kDa |
| Observed MW: | 55kDa |
| Synonyms: | CDC20, CDC20A, bA276H19.3, p55CDC |
| Background: | CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation. |
| UniProt Protein Function: | CDC20: Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation. Found in a complex with CDC20, CDC27, SPATC1 and TUBG1. Interacts with SPATC1. Interacts with NEUROD2. Interacts with MAD2L1 and BUB1B. The phosphorylated form interacts with APC/C. Interacts with NINL. May interact with MAD2L2. Interacts with CDK5RAP2. Interacts with isoform 1 of NEK2. Belongs to the WD repeat CDC20/Fizzy family. |
| UniProt Protein Details: | Protein type:Ubiquitin conjugating system; Cell cycle regulation Chromosomal Location of Human Ortholog: 1p34.1 Cellular Component: anaphase-promoting complex; cytoplasm; cytosol; nucleoplasm; nucleus; spindle Molecular Function:enzyme binding; protein binding; protein C-terminus binding Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of synaptic plasticity; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; sister chromatid cohesion |
| NCBI Summary: | CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q12834 |
| NCBI GenInfo Identifier: | 37537762 |
| NCBI Gene ID: | 991 |
| NCBI Accession: | Q12834.2 |
| UniProt Secondary Accession: | Q12834,Q5JUY4, Q9BW56, Q9UQI9, B2R6Z6, D3DPJ1, |
| UniProt Related Accession: | Q12834 |
| Molecular Weight: | 54,723 Da |
| NCBI Full Name: | Cell division cycle protein 20 homolog |
| NCBI Synonym Full Names: | cell division cycle 20 |
| NCBI Official Symbol: | CDC20 |
| NCBI Official Synonym Symbols: | CDC20A; p55CDC; bA276H19.3 |
| NCBI Protein Information: | cell division cycle protein 20 homolog |
| UniProt Protein Name: | Cell division cycle protein 20 homolog |
| UniProt Synonym Protein Names: | p55CDC |
| Protein Family: | Cell division cycle protein |
| UniProt Gene Name: | CDC20 |
| UniProt Entry Name: | CDC20_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CDC20 antibody (CAB1231) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
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