Immunology Antibodies 2

Anti-CD8B Antibody (CAB3286)

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SKU:
CAB3286
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-CD8B Antibody
Antibody SKU:CAB3286
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 22-170 of human CD8B (NP_757362.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, Mouse thymus, Mouse liver, Mouse kidney, Rat thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 22-170 of human CD8B (NP_757362.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LQQT PAYI KVQT NKMV MLSC EAKI SLSN MRIY WLRQ RQAP SSDS HHEF LALW DSAK GTIH GEEV EQEK IAVF RDAS RFIL NLTS VKPE DSGI YFCM IVGS PELT FGKG TQLS VVDF LPTT AQPT KKST LKKR VCRL PRPE TQKG PLCS P
Gene ID:926
Uniprot:P10966
Cellular Location:Cell membrane, Secreted, Single-pass type I membrane protein, Single-pass type I membrane protein
Calculated MW:20kDa/22kDa/23kDa/24kDa/25kDa/27kDa
Observed MW:45kDa
Synonyms:CD8B, CD8B1, LEU2, LY3, LYT3, P37
Background:The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified.
UniProt Protein Function:CD8B: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. 8 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral

Chromosomal Location of Human Ortholog: 2p12

Cellular Component: T cell receptor complex; integral to plasma membrane; early endosome membrane; plasma membrane; extracellular region; external side of plasma membrane

Molecular Function:protein binding; MHC class I protein binding; coreceptor activity

Biological Process: regulation of immune response; T cell activation; viral reproduction; immune response; regulation of defense response to virus by virus; transmembrane receptor protein tyrosine kinase signaling pathway

NCBI Summary:The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified. [provided by RefSeq, May 2010]
UniProt Code:P10966
NCBI GenInfo Identifier:116032
NCBI Gene ID:926
NCBI Accession:P10966.1
UniProt Secondary Accession:P10966,P14860, P14861, Q15980, Q496E0, Q496E1, Q496E2 Q9UDB4, Q9UDB5, Q9UDB6, Q9UDB7, Q9UDB8,
UniProt Related Accession:P10966
Molecular Weight:210
NCBI Full Name:T-cell surface glycoprotein CD8 beta chain
NCBI Synonym Full Names:CD8b molecule
NCBI Official Symbol:CD8B  
NCBI Official Synonym Symbols:LY3; P37; LEU2; LYT3; CD8B1  
NCBI Protein Information:T-cell surface glycoprotein CD8 beta chain; CD8 antigen, beta polypeptide 1 (p37); T lymphocyte surface glycoprotein beta chain
UniProt Protein Name:T-cell surface glycoprotein CD8 beta chain
UniProt Synonym Protein Names:CD_antigen: CD8b
Protein Family:T-cell surface glycoprotein
UniProt Gene Name:CD8B  
UniProt Entry Name:CD8B_HUMAN
Anti-CD8B Antibody (CAB3286)Western blot analysis of extracts of various cell lines, using CD8B antibody (CAB3286) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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