Name: Anti-CD207 Antibody (CAB6553)
Brand: Cell Biology Antibodies 10
SKU: CAB6553
Availability: OutOfStock

Antibody Name:	Anti-CD207 Antibody
Antibody SKU:	CAB6553
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 69-328 of human CD207 (NP_056532.4).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	HT-29, HepG2, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 69-328 of human CD207 (NP_056532.4).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GTIS DVKT NVQL LKGR VDNI STLD SEIK KNSD GMEA AGVQ IQMV NESL GYVR SQFL KLKT SVEK ANAQ IQIL TRSW EEVS TLNA QIPE LKSD LEKA SALN TKIR ALQG SLEN MSKL LKRQ NDIL QVVS QGWK YFKG NFYY FSLI PKTW YSAE QFCV SRNS HLTS VTSE SEQE FLYK TAGG LIYW IGLT KAGM EGDW SWVD DTPF NKVQ SVRF WIPG EPNN AGNN EHCG NIKA PSLQ AWND APCD KTFL FICK RPYV PSEP
Gene ID:	50489
Uniprot:	Q9UJ71
Cellular Location:	Membrane, Single-pass type II membrane protein
Calculated MW:	36kDa
Observed MW:	37kDa

Synonyms:

CD207, CLEC4K

Background:

The protein encoded by this gene is expressed only in Langerhans cells which are immature dendritic cells of the epidermis and mucosa. It is localized in the Birbeck granules, organelles present in the cytoplasm of Langerhans cells and consisting of superimposed and zippered membranes. It is a C-type lectin with mannose binding specificity, and it has been proposed that mannose binding by this protein leads to internalization of antigen into Birbeck granules and providing access to a nonclassical antigen-processing pathway. Mutations in this gene result in Birbeck granules deficiency or loss of sugar binding activity.

UniProt Protein Function:	langerin: Calcium-dependent lectin displaying mannose-binding specificity. Induces the formation of Birbeck granules (BGs); is a potent regulator of membrane superimposition and zippering. Binds to sulfated as well as mannosylated glycans, keratan sulfate (KS) and beta-glucans. Facilitates uptake of antigens and is involved in the routing and/or processing of antigen for presentation to T cells. Major receptor on primary Langerhans cells for Candida species, Saccharomyces species, and Malassezia furfur. Protects against human immunodeficiency virus-1 (HIV-1) infection. Binds to high-mannose structures present on the envelope glycoprotein which is followed by subsequent targeting of the virus to the Birbeck granules leading to its rapid degradation. Homotrimer. Exclusively expressed by Langerhans cells. Expressed in astrocytoma and malignant ependymoma, but not in normal brain tissues.
UniProt Protein Details:	Protein type:Receptor, misc.; Membrane protein, integral Chromosomal Location of Human Ortholog: 2p13 Cellular Component: endocytic vesicle; early endosome membrane; plasma membrane; integral to membrane Molecular Function:mannose binding; carbohydrate binding Biological Process: antigen processing and presentation of peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I; defense response to virus Disease: Birbeck Granule Deficiency
NCBI Summary:	The protein encoded by this gene is expressed only in Langerhans cells which are immature dendritic cells of the epidermis and mucosa. It is localized in the Birbeck granules, organelles present in the cytoplasm of Langerhans cells and consisting of superimposed and zippered membranes. It is a C-type lectin with mannose binding specificity, and it has been proposed that mannose binding by this protein leads to internalization of antigen into Birbeck granules and providing access to a nonclassical antigen-processing pathway. Mutations in this gene result in Birbeck granules deficiency or loss of sugar binding activity. [provided by RefSeq, Aug 2010]
UniProt Code:	Q9UJ71
NCBI GenInfo Identifier:	229784129
NCBI Gene ID:	50489
NCBI Accession:	Q9UJ71.2
UniProt Related Accession:	Q9UJ71
Molecular Weight:	328
NCBI Full Name:	C-type lectin domain family 4 member K
NCBI Synonym Full Names:	CD207 molecule, langerin
NCBI Official Symbol:	CD207
NCBI Official Synonym Symbols:	CLEC4K
NCBI Protein Information:	C-type lectin domain family 4 member K; CD207 antigen, langerin; Langerhans cell specific c-type lectin; C-type lectin domain family 4, member K
UniProt Protein Name:	C-type lectin domain family 4 member K
UniProt Synonym Protein Names:	Langerin; CD_antigen: CD207
Protein Family:	C-type lectin domain family
UniProt Gene Name:	CD207
UniProt Entry Name:	CLC4K_HUMAN

Western blot analysis of extracts of various cell lines, using CD207 antibody (CAB6553) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

CD207 Antibody (PACO01976)

CD207 Antibody (PACO08273)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)