Immunology Antibodies 2
Anti-CD14 Antibody (CAB5737)
- SKU:
- CAB5737
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-CD14 Antibody |
| Antibody SKU: | CAB5737 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-330 of human CD14 (NP_001167576.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse lung, Mouse spleen, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-330 of human CD14 (NP_001167576.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TTPE PCEL DDED FRCV CNFS EPQP DWSE AFQC VSAV EVEI HAGG LNLE PFLK RVDA DADP RQYA DTVK ALRV RRLT VGAA QVPA QLLV GALR VLAY SRLK ELTL EDLK ITGT MPPL PLEA TGLA LSSL RLRN VSWA TGRS WLAE LQQW LKPG LKVL SIAQ AHSP AFSC EQVR AFPA LTSL DLSD NPGL GERG LMAA LCPH KFPA IQNL ALRN TGME TPTG VCAA LAAA GVQP HSLD LSHN SLRA TVNP SAPR CMWS SALN SLNL SFAG LEQV PKGL PAKL RVLD LSCN RLNR APQP DELP EVDN LTLD GNP |
| Gene ID: | 929 |
| Uniprot: | P08571 |
| Cellular Location: | Cell membrane, GPI-anchor, Lipid-anchor, Secreted |
| Calculated MW: | 40kDa |
| Observed MW: | 70kDa |
| Synonyms: | CD14 |
| Background: | The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. |
| UniProt Protein Function: | CD14: Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. |
| UniProt Protein Details: | Protein type:Membrane protein, GPI anchor Cellular Component: anchored to external side of plasma membrane; extracellular space; cell surface; membrane; plasma membrane; lipopolysaccharide receptor complex; lipid raft; external side of plasma membrane Molecular Function:lipopolysaccharide binding Biological Process: response to bacterium; immune system process; response to molecule of bacterial origin; innate immune response; positive regulation of endocytosis; positive regulation of tumor necrosis factor production; inflammatory response; positive regulation of cytokine secretion |
| NCBI Summary: | This gene encodes a protein that plays an important role in the innate immune response and is expressed in monocyte/macrophage cells. This gene product acts as a co-receptor that binds several microbial and fungal molecules, including lipopolysaccharides (LPS). This proteins LPS-binding activity is enhanced by the LPS binding protein (LBP) to allow binding to the TLR4-MD-2 co-receptor complex. The product of this gene is found in two forms, either as a soluble protein or attached to the cell surface by a glycosylphosphatidylinositol anchor. [provided by RefSeq, Jul 2014] |
| UniProt Code: | P08571 |
| NCBI GenInfo Identifier: | 6753332 |
| NCBI Gene ID: | 12475 |
| NCBI Accession: | NP_033971.1 |
| UniProt Related Accession: | P10810 |
| Molecular Weight: | |
| NCBI Full Name: | monocyte differentiation antigen CD14 |
| NCBI Synonym Full Names: | CD14 antigen |
| NCBI Official Symbol: | Cd14 |
| NCBI Protein Information: | monocyte differentiation antigen CD14 |
| UniProt Protein Name: | Monocyte differentiation antigen CD14 |
| UniProt Synonym Protein Names: | Myeloid cell-specific leucine-rich glycoprotein; CD_antigen: CD14 |
| Protein Family: | Monocyte differentiation antigen |
| UniProt Gene Name: | Cd14 |
| UniProt Entry Name: | CD14_MOUSE |
 | Western blot analysis of extracts of various cell lines, using CD14 antibody (CAB5737) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded human spleen using CD14 antibody (CAB5737) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of RAW264.7 cells using CD14 antibody (CAB5737) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of THP-1 cells using CD14 antibody (CAB5737) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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