Name: Anti-CCNDBP1 Antibody (CAB13735)
Brand: Cell Cycle Antibodies 1
SKU: CAB13735
Availability: OutOfStock

Antibody Name:	Anti-CCNDBP1 Antibody
Antibody SKU:	CAB13735
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-360 of human CCNDBP1 (NP_036274.3).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	LO2, 22Rv1, A-549, BxPC-3, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-360 of human CCNDBP1 (NP_036274.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MASA TAPA AAVP TLAS PLEQ LRHL AEEL RLLL PRVR VGEA QETT EEFN REMF WRRL NEAA VTVS REAT TLTI VFSQ LPLP SPQE TQKF CEQV HAAI KAFI AVYY LLPK DQGI TLRK LVRG ATLD IVDG MAQL MEVL SVTP TQSP ENND LISY NSVW VACQ QMPQ IPRD NKAA ALLM LTKN VDFV KDAH EEME QAVE ECDP YSGL LNDT EENN SDNH NHED DVLG FPSN QDLY WSED DQEL IIPC LALV RASK ACLK KIRM LVAE NGKK DQVA QLDD IVDI SDEI SPSV DDLA LSIY PPMC HLTV RINS AKLV SVLK KALE ITKA SHVT PQPE DSWI PLLI NAID HCMN RIKE LTQS ELEL
Gene ID:	23582
Uniprot:	O95273
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	22kDa/26kDa/34kDa/40kDa
Observed MW:	42kDa

Synonyms:

CCNDBP1, DIP1, GCIP, HHM

Background:

This gene was identified by the interaction of its gene product with Grap2, a leukocyte-specific adaptor protein important for immune cell signaling. The protein encoded by this gene was shown to interact with cyclin D. Transfection of this gene in cells was reported to reduce the phosphorylation of Rb gene product by cyclin D-dependent protein kinase, and inhibit E2F1-mediated transcription activity. This protein was also found to interact with helix-loop-helix protein E12 and is thought to be a negative regulator of liver-specific gene expression. Several alternatively spliced variants have been found for this gene.

UniProt Protein Function:	CCNDBP1: May negatively regulate cell cycle progression. May act at least in part via inhibition of the cyclin-D1/CDK4 complex, thereby preventing phosphorylation of RB1 and blocking E2F- dependent transcription. Belongs to the CCNDBP1 family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 15q14-q15 Cellular Component: nucleoplasm; nucleus Molecular Function:protein binding
NCBI Summary:	This gene was identified by the interaction of its gene product with Grap2, a leukocyte-specific adaptor protein important for immune cell signaling. The protein encoded by this gene was shown to interact with cyclin D. Transfection of this gene in cells was reported to reduce the phosphorylation of Rb gene product by cyclin D-dependent protein kinase, and inhibit E2F1-mediated transcription activity. This protein was also found to interact with helix-loop-helix protein E12 and is thought to be a negative regulator of liver-specific gene expression. Several alternatively spliced variants have been found for this gene. [provided by RefSeq, Apr 2009]
UniProt Code:	O95273
NCBI GenInfo Identifier:	172047302
NCBI Gene ID:	23582
NCBI Accession:	O95273.2
UniProt Secondary Accession:	O95273,Q6ZQN9, Q7Z519, Q8NBS7, Q8NBY2, Q9NS19, Q9NYH3 Q9UHX9, A8K3Q0, A8K3U2,
UniProt Related Accession:	O95273
Molecular Weight:	22,714 Da
NCBI Full Name:	Cyclin-D1-binding protein 1
NCBI Synonym Full Names:	cyclin D1 binding protein 1
NCBI Official Symbol:	CCNDBP1
NCBI Official Synonym Symbols:	HHM; DIP1; GCIP
NCBI Protein Information:	cyclin-D1-binding protein 1
UniProt Protein Name:	Cyclin-D1-binding protein 1
UniProt Synonym Protein Names:	Grap2 and cyclin-D-interacting protein; Human homolog of Maid
Protein Family:	Cyclin-D1-binding protein
UniProt Gene Name:	CCNDBP1
UniProt Entry Name:	CCDB1_HUMAN

Western blot analysis of extracts of various cell lines, using CCNDBP1 antibody (CAB13735) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

CCNDBP1 Antibody (PACO08248)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)