Cell Biology Antibodies 11
Anti-CCL3 Antibody (CAB7568)
- SKU:
- CAB7568
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-CCL3 Antibody |
| Antibody SKU: | CAB7568 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 24-92 of human CCL3 (NP_002974.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | THP-1 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 24-92 of human CCL3 (NP_002974.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SLAA DTPT ACCF SYTS RQIP QNFI ADYF ETSS QCSK PGVI FLTK RSRQ VCAD PSEE WVQK YVSD LELS A |
| Gene ID: | 6348 |
| Uniprot: | P10147 |
| Cellular Location: | Secreted |
| Calculated MW: | 10kDa |
| Observed MW: | 11KDa |
| Synonyms: | CCL3, G0S19-1, LD78ALPHA, MIP-1-alpha, MIP1A, SCYA3 |
| Background: | This locus represents a small inducible cytokine. The encoded protein, also known as macrophage inflammatory protein 1 alpha, plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. Polymorphisms at this locus may be associated with both resistance and susceptibility to infection by human immunodeficiency virus type 1. |
| UniProt Protein Function: | CCL3: Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Belongs to the intercrine beta (chemokine CC) family. |
| UniProt Protein Details: | Protein type:Chemokine; Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 17q12 Cellular Component: extracellular space; cytoplasm; extracellular region; intracellular; cytosol Molecular Function:identical protein binding; CCR1 chemokine receptor binding; protein binding; chemokine activity; calcium-dependent protein kinase C activity; kinase activity; protein kinase activity; CCR5 chemokine receptor binding; chemoattractant activity; phospholipase activator activity Biological Process: positive regulation of catalytic activity; negative regulation of osteoclast differentiation; exocytosis; response to toxin; behavior; positive regulation of calcium-mediated signaling; positive regulation of interleukin-1 beta secretion; chemotaxis; protein amino acid phosphorylation; regulation of cell shape; negative regulation of bone mineralization; monocyte chemotaxis; positive chemotaxis; cell-cell signaling; calcium ion transport; positive regulation of neuron apoptosis; protein kinase B signaling cascade; lipopolysaccharide-mediated signaling pathway; inflammatory response; lymphocyte chemotaxis; neutrophil chemotaxis; MAPKKK cascade; calcium-mediated signaling; cytoskeleton organization and biogenesis; release of sequestered calcium ion by sarcoplasmic reticulum into cytosol; positive regulation of tumor necrosis factor production; macrophage chemotaxis; cellular calcium ion homeostasis; osteoblast differentiation; cell activation; positive regulation of protein kinase B signaling cascade; eosinophil chemotaxis; eosinophil degranulation; regulation of sensory perception of pain; positive regulation of calcium ion transport; astrocyte cell migration; positive regulation of inflammatory response; positive regulation of cell migration Disease: Human Immunodeficiency Virus Type 1, Susceptibility To |
| NCBI Summary: | This locus represents a small inducible cytokine. The encoded protein, also known as macrophage inflammatory protein 1 alpha, plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. Polymorphisms at this locus may be associated with both resistance and susceptibility to infection by human immunodeficiency virus type 1.[provided by RefSeq, Sep 2010] |
| UniProt Code: | P10147 |
| NCBI GenInfo Identifier: | 127078 |
| NCBI Gene ID: | 6348 |
| NCBI Accession: | P10147.1 |
| UniProt Related Accession: | P10147 |
| Molecular Weight: | 10,085 Da |
| NCBI Full Name: | C-C motif chemokine 3 |
| NCBI Synonym Full Names: | chemokine (C-C motif) ligand 3 |
| NCBI Official Symbol: | CCL3 |
| NCBI Official Synonym Symbols: | MIP1A; SCYA3; G0S19-1; LD78ALPHA; MIP-1-alpha |
| NCBI Protein Information: | C-C motif chemokine 3; SIS-beta; PAT 464.1; G0/G1 switch regulatory protein 19-1; macrophage inflammatory protein 1-alpha; tonsillar lymphocyte LD78 alpha protein; small inducible cytokine A3 (homologous to mouse Mip-1a) |
| UniProt Protein Name: | C-C motif chemokine 3 |
| UniProt Synonym Protein Names: | G0/G1 switch regulatory protein 19-1; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; PAT 464.1; SIS-beta; Small-inducible cytokine A3; Tonsillar lymphocyte LD78 alpha proteinCleaved into the following chain:MIP-1-alpha(4-69)Alternative name(s):LD78-alpha(4-69) |
| UniProt Gene Name: | CCL3 |
| UniProt Entry Name: | CCL3_HUMAN |
 | Western blot analysis of extracts of THP-1 cells, using CCL3 antibody (CAB7568) at 1:1000 dilution.THP-1 cells were treated by LPS for 6 hours and Brefeldin A for 3 hours of stimulation. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s. |
 | Immunohistochemistry of paraffin-embedded human liver using CCL3 antibody (CAB7568) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human gastric cancer using CCL3 antibody (CAB7568) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using CCL3 Rabbit pAb (CAB7568) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH-3T3 cells using CCL3 Rabbit pAb (CAB7568) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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