Epigenetics & Nuclear Signaling Antibodies 3

Anti-CBX4 Antibody (CAB5532)

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SKU:
CAB5532
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-CBX4 Antibody
Antibody SKU:CAB5532
Antibody Size:20uL, 50uL, 100uL
Application:WB IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 401-560 of human CBX4 (NP_003646.2).
Application:WB IP
Recommended Dilution:WB 1:500 - 1:2000 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, HepG2, SW620, Jurkat, Mouse brain, Mouse liver, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 401-560 of human CBX4 (NP_003646.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AVGL NLSH VRKR CLSE THGE REPC KKRL TARS ISTP TCLG GSPA AERP ADLP PAAA LPQP EVIL LDSD LDEP IDLR CVKT RSEA GEPP SSLQ VKPE TPAS AAVA VAAA AAPT TTAE KPPA EAQD EPAE SLSE FKPF FGNI IITD VTAN CLTV TFKE YVTV
Gene ID:8535
Uniprot:O00257
Cellular Location:Nucleus, Nucleus speckle
Calculated MW:31kDa/61kDa
Observed MW:73kDa
Synonyms:CBX4, NBP16, PC2
Background:
UniProt Protein Function:CBX4: E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 6.3.2.-; SUMO ligase; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: nucleoplasm; nucleus; PcG protein complex

Molecular Function:enzyme binding; protein binding; SUMO binding; SUMO ligase activity; transcription corepressor activity

Biological Process: negative regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; protein sumoylation

UniProt Code:O00257
NCBI GenInfo Identifier:254763424
NCBI Gene ID:8535
NCBI Accession:O00257.3
UniProt Secondary Accession:O00257,Q6TPI8, Q96C04, B1PJR7,
UniProt Related Accession:O00257
Molecular Weight:31,987 Da
NCBI Full Name:E3 SUMO-protein ligase CBX4
NCBI Synonym Full Names:chromobox 4
NCBI Official Symbol:CBX4  
NCBI Official Synonym Symbols:PC2; NBP16  
NCBI Protein Information:E3 SUMO-protein ligase CBX4
UniProt Protein Name:E3 SUMO-protein ligase CBX4
UniProt Synonym Protein Names:Chromobox protein homolog 4; Polycomb 2 homolog; Pc2; hPc2
Protein Family:E3 SUMO-protein ligase
UniProt Gene Name:CBX4  
UniProt Entry Name:CBX4_HUMAN
Anti-CBX4 Antibody (CAB5532)Western blot analysis of extracts of various cell lines, using CBX4 antibody (CAB5532) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-CBX4 Antibody (CAB5532)Immunoprecipitation analysis of 200ug extracts of HepG2 cells using 1ug CBX4 antibody (CAB5532). Western blot was performed from the immunoprecipitate using CBX4 antibody (CAB5532) at a dilition of 1:500.
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