Antibody Name:	Anti-CBX3 Antibody [KO Validated]
Antibody SKU:	CAB2248
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human CBX3 (NP_009207.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, MCF-7, 293T, A-549, NCI-H460, Mouse brain, Mouse lung, Mouse spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human CBX3 (NP_009207.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MASN KTTL QKMG KKQN GKSK KVEE AEPE EFVV EKVL DRRV VNGK VEYF LKWK GFTD ADNT WEPE ENLD CPEL IEAF LNSQ KAGK EKDG TKRK SLSD SESD DSKS KKKR DAAD KPRG FARG LDPE RIIG ATDS SGEL MFLM KWKD SDEA DLVL AKEA NMKC PQIV IAFY EERL TWHS CPED EAQ
Gene ID:	11335
Uniprot:	Q13185
Cellular Location:	Nucleus
Calculated MW:	20kDa
Observed MW:	23kDa

Synonyms:

CBX3, HECH, HP1-GAMMA, HP1Hs-gamma

Background:

At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.

UniProt Protein Function:	CBX3: Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Binds directly to CHAF1A. Interacts with histone H3 methylated at 'Lys-9'. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Interacts with LBR, INCENP, TRIM28/TIF1B, SUV420H1, SUV420H2 and SP100. Interacts with TIF1A. Interacts with MIS12 and DSN1. Can interact directly with CBX5 via the chromoshadow domain. Interacts with POGZ. Interacts with CHAMP1. Interacts with ASXL1.
UniProt Protein Details:	Protein type:DNA-binding Chromosomal Location of Human Ortholog: 7p15.2 Cellular Component: chromatin; chromocenter; chromosome, pericentric region; nuclear chromosome, telomeric region; nuclear envelope; nuclear heterochromatin; nuclear inner membrane; nucleus; spindle Molecular Function:enzyme binding; identical protein binding; protein binding; protein domain specific binding Biological Process: chromatin remodeling; negative regulation of transcription, DNA-dependent; rhythmic process; transcription, DNA-dependent
NCBI Summary:	At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.[provided by RefSeq, Mar 2011]
UniProt Code:	Q13185
NCBI GenInfo Identifier:	116241284
NCBI Gene ID:	11335
NCBI Accession:	Q13185.4
UniProt Secondary Accession:	Q13185,Q96CD7, Q99409, Q9BVS3, Q9P0Z6,
UniProt Related Accession:	Q13185
Molecular Weight:	20,811 Da
NCBI Full Name:	Chromobox protein homolog 3
NCBI Synonym Full Names:	chromobox 3
NCBI Official Symbol:	CBX3
NCBI Official Synonym Symbols:	HECH; HP1-GAMMA; HP1Hs-gamma
NCBI Protein Information:	chromobox protein homolog 3
UniProt Protein Name:	Chromobox protein homolog 3
UniProt Synonym Protein Names:	HECH; Heterochromatin protein 1 homolog gamma; HP1 gamma; Modifier 2 protein
Protein Family:	Chromobox protein
UniProt Gene Name:	CBX3
UniProt Entry Name:	CBX3_HUMAN

	Western blot analysis of extracts of various cell lines, using CBX3 antibody (CAB2248) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Western blot analysis of extracts from normal (control) and CBX3 knockout (KO) HeLa cells, using CBX3 antibody (CAB2248) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded rat brain using [KO Validated] CBX3 Rabbit pAb (CAB2248) at dilution of 1:50 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal cancer using [KO Validated] CBX3 Rabbit pAb (CAB2248) at dilution of 1:50 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse liver using [KO Validated] CBX3 Rabbit pAb (CAB2248) at dilution of 1:50 (40x lens).

Antibodies	ELISA Kits
Anti-Phospho-CBX3-S83 pAb Antibody (CABP0801)	Human CBX3 / Chromobox Homolog 3 ELISA Kit
Anti-HP1 gamma Antibody (CAB4283)	Human CBX3 (Chromobox Homolog 3) ELISA Kit (HUES01881)
CBX3 Antibody (PACO02423)	HP1gamma Colorimetric Cell-Based ELISA Kit
Phospho-CBX3 (S93) Antibody (PACO03393)	HP1gamma (Phospho-Ser93) Colorimetric Cell-Based ELISA Kit
CBX3 Antibody (PACO08216)