Name: Anti-CBX2 Antibody (CAB2683)
Brand: Developmental Biology
SKU: CAB2683
Availability: OutOfStock

Antibody Name:	Anti-CBX2 Antibody
Antibody SKU:	CAB2683
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 62-211 of human CBX2 (NP_116036.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	HeLa, K-562, MCF7, BT-474

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 62-211 of human CBX2 (NP_116036.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EHEK EVQN RKRG KRPR GRPR KLTA MSSC SRRS KLKV GGCA GYAD PTSQ HPLG VGGR QREG LGPS GRGW HFCQ QSVP LLGK QEPP FFLS LSFC CQGP QPAE SSSP PLPG ASCF SLSC TPLC WVAG SNCC RQAL FPPR GSLG DGKE QEAC VQ
Gene ID:	84733
Uniprot:	Q14781
Cellular Location:	Nucleus
Calculated MW:	23kDa/56kDa
Observed MW:	65kDa

Synonyms:

CBX2, CDCA6, M33, SRXY5

Background:

This gene encodes a component of the polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. Disruption of this gene in mice results in male-to-female gonadal sex reversal. Mutations in this gene are also associated with gonadal dysgenesis in humans. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

UniProt Protein Function:	CBX2: Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Involved in sexual development, acting as activator of NR5A1 expression. Defects in CBX2 are the cause of 46,XY sex reversal type 5 (SRXY5). It is a disorder of sex development. Affected individuals have a 46,XY karyotype but present as phenotypically normal females. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transcription regulation Chromosomal Location of Human Ortholog: 17q25.3 Cellular Component: nucleoplasm; nucleus; PcG protein complex Molecular Function:protein binding Biological Process: development of primary sexual characteristics; negative regulation of transcription from RNA polymerase II promoter; protein sumoylation Disease: 46,xy Sex Reversal 5
NCBI Summary:	This gene encodes a component of the polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. Disruption of this gene in mice results in male-to-female gonadal sex reversal. Mutations in this gene are also associated with gonadal dysgenesis in humans. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Mar 2010]
UniProt Code:	Q14781
NCBI GenInfo Identifier:	77416853
NCBI Gene ID:	84733
NCBI Accession:	Q14781.2
UniProt Secondary Accession:	Q14781,Q0VDA5, Q9BTB1,
UniProt Related Accession:	Q14781
Molecular Weight:	23,312 Da
NCBI Full Name:	Chromobox protein homolog 2
NCBI Synonym Full Names:	chromobox 2
NCBI Official Symbol:	CBX2
NCBI Official Synonym Symbols:	M33; CDCA6; SRXY5
NCBI Protein Information:	chromobox protein homolog 2
UniProt Protein Name:	Chromobox protein homolog 2
Protein Family:	Chromobox protein
UniProt Gene Name:	CBX2
UniProt Entry Name:	CBX2_HUMAN

Western blot analysis of extracts of various cell lines, using CBX2 antibody (CAB2683) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

CBX2 Antibody (PACO05538)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)