KO Validated Antibodies 1

Anti-CBR1 Antibody (CAB18074)[KO Validated]

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SKU:
CAB18074
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CBR1 Antibody [KO Validated]
Antibody SKU:CAB18074
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-277 of human CBR1 (NP_001748.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:Human, Mouse
Positive Samples:A-549
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-277 of human CBR1 (NP_001748.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSSG IHVA LVTG GNKG IGLA IVRD LCRL FSGD VVLT ARDV TRGQ AAVQ QLQA EGLS PRFH QLDI DDLQ SIRA LRDF LRKE YGGL DVLV NNAG IAFK VADP TPFH IQAE VTMK TNFF GTRD VCTE LLPL IKPQ GRVV NVSS IMSV RALK SCSP ELQQ KFRS ETIT EEEL VGLM NKFV EDTK KGVH QKEG WPSS AYGV TKIG VTVL SRIH ARKL SEQR KGDK ILLN ACCP GWVR TDMA GPKA TKSP EEGA ETPV YLAL LPPD AEGP HGQF VSEK RVEQ W
Gene ID:873
Uniprot:P16152
Cellular Location:Cytoplasm
Calculated MW:18kDa/30kDa
Observed MW:30kDa
Anti-CBR1 Antibody [KO Validated] (CAB18074)Western blot analysis of extracts from normal (control) and CBR1 knockout (KO) A-549 cells, using CBR1 antibody (CAB18074) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Synonyms:CBR1, CBR, SDR21C1, hCBR1
Background:The protein encoded by this gene belongs to the short-chain dehydrogenases/reductases (SDR) family, which function as NADPH-dependent oxidoreductases having wide specificity for carbonyl compounds, such as quinones, prostaglandins, and various xenobiotics. Alternatively spliced transcript variants have been found for this gene.
UniProt Protein Function:CBR1: NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E2 to prostaglandin F2-alpha. Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione. Belongs to the short-chain dehydrogenases/reductases (SDR) family.
UniProt Protein Details:

Protein type:EC 1.1.1.189; Lipid Metabolism - arachidonic acid; EC 1.1.1.197; Oxidoreductase; EC 1.1.1.184

Chromosomal Location of Human Ortholog: 21q22.13

Cellular Component: cytosol; vesicle

Molecular Function:oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; prostaglandin-E2 9-reductase activity; 15-hydroxyprostaglandin dehydrogenase (NADP+) activity; carbonyl reductase (NADPH) activity

Biological Process: epithelial cell differentiation; vitamin K metabolic process; cyclooxygenase pathway; arachidonic acid metabolic process; drug metabolic process

NCBI Summary:The protein encoded by this gene belongs to the short-chain dehydrogenases/reductases (SDR) family, which function as NADPH-dependent oxidoreductases having wide specificity for carbonyl compounds, such as quinones, prostaglandins, and various xenobiotics. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2013]
UniProt Code:P16152
NCBI GenInfo Identifier:118519
NCBI Gene ID:873
NCBI Accession:P16152.3
UniProt Secondary Accession:P16152,Q3LHW8, B2RBZ7, B4DFK7,
UniProt Related Accession:P16152
Molecular Weight:277
NCBI Full Name:Carbonyl reductase
NCBI Synonym Full Names:carbonyl reductase 1
NCBI Official Symbol:CBR1  
NCBI Official Synonym Symbols:CBR; hCBR1; SDR21C1  
NCBI Protein Information:carbonyl reductase [NADPH] 1; carbonyl reductase [NADPH] 1; carbonyl reductase (NADPH) 1; prostaglandin 9-ketoreductase; prostaglandin-E(2) 9-reductase; NADPH-dependent carbonyl reductase 1; 15-hydroxyprostaglandin dehydrogenase; short chain dehydrogenase/reductase family 21C, member 1
UniProt Protein Name:Carbonyl reductase [NADPH] 1
UniProt Synonym Protein Names:15-hydroxyprostaglandin dehydrogenase [NADP(+)] (EC:1.1.1.197); NADPH-dependent carbonyl reductase 1; Prostaglandin 9-ketoreductase; Prostaglandin-E(2) 9-reductase (EC:1.1.1.189)
Protein Family:Carbonyl reductase
UniProt Gene Name:CBR1  
UniProt Entry Name:CBR1_HUMAN
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