Name: Anti-CBP80 Antibody (CAB13939)
Brand: Epigenetics & Nuclear Signaling Antibodies 1
SKU: CAB13939
Availability: OutOfStock

Antibody Name:	Anti-CBP80 Antibody
Antibody SKU:	CAB13939
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	A synthetic peptide of human CBP80

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	NIH/3T3, Mouse testis, Mouse brain

Immunogen:	A synthetic peptide of human CBP80
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	4686
Uniprot:	Q09161
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	91kDa
Observed MW:	100KDa

Synonyms:	NCBP1, CBP80, NCBP, Sto1
Background:	The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay.

UniProt Protein Function:	NCBP1: mediates U snRNA export from the nucleus. Binds to 5' capped mRNA. Found in aU snRNA export complex with RNUXA/PHAX, CBP20, CBP80, RAN, XPO1 and m7G-capped RNA. Interaction with RNUXA/PHAX. Heterodimer with NCBP2. Is part of the exon junction complex (EJC) containing NCBP1, NCBP2, RNPS1, RBM8A, SRRM1, NXF1, UPF3B, UPF2, THOC4 and/or REFBP2.
UniProt Protein Details:	Protein type:Translation; RNA processing; Spliceosome Chromosomal Location of Human Ortholog: 9q34.1 Cellular Component: cytoplasm; cytosol; mitochondrion; mRNA cap complex; nucleoplasm; nucleus; ribonucleoprotein complex Molecular Function:protein binding; RNA binding; RNA cap binding Biological Process: gene expression; histone mRNA metabolic process; mRNA 3'-end processing; mRNA capping; mRNA catabolic process, nonsense-mediated decay; mRNA export from nucleus; nuclear mRNA cis splicing, via U2-type spliceosome; nuclear mRNA splicing, via spliceosome; positive regulation of cell growth; positive regulation of mRNA 3'-end processing; positive regulation of viral transcription; regulation of translational initiation; RNA elongation from RNA polymerase II promoter; RNA splicing; RNA-mediated gene silencing; spliceosomal snRNP biogenesis; termination of RNA polymerase II transcription; transcription from RNA polymerase II promoter; viral reproduction
NCBI Summary:	The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. [provided by RefSeq, Jul 2008]
UniProt Code:	Q09161
NCBI GenInfo Identifier:	1705654
NCBI Gene ID:	4686
NCBI Accession:	Q09161.1
UniProt Secondary Accession:	Q09161,Q59G76, Q5T1V0, Q5T7X2, B2R718,
UniProt Related Accession:	Q09161
Molecular Weight:	91,839 Da
NCBI Full Name:	Nuclear cap-binding protein subunit 1
NCBI Synonym Full Names:	nuclear cap binding protein subunit 1
NCBI Official Symbol:	NCBP1
NCBI Official Synonym Symbols:	NCBP; Sto1; CBP80
NCBI Protein Information:	nuclear cap-binding protein subunit 1
UniProt Protein Name:	Nuclear cap-binding protein subunit 1
UniProt Synonym Protein Names:	80 kDa nuclear cap-binding protein; CBP80; NCBP 80 kDa subunit
Protein Family:	CBP80/20-dependent translation initiation factor
UniProt Gene Name:	NCBP1
UniProt Entry Name:	NCBP1_HUMAN

	Western blot analysis of extracts of various cell lines, using CBP80 antibody (CAB13939) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Western blot analysis of extracts of various cell lines, using CBP80 antibody (CAB13939) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded human brain using CBP80 Rabbit pAb (CAB13939) at dilution of 1:100 (40x lens).