Anti-CASTOR1 Antibody (CAB20710)
- SKU:
- CAB20710
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
| Antibody Name: | Anti-CASTOR1 Antibody (CAB20710) |
| Antibody SKU: | CAB20710 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting, Immunohistochemistry, Immunofluorescence |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-329 of human CASTOR1 (NP_001032755.1). |
| Application: | Western blotting, Immunohistochemistry, Immunofluorescence |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-329 of human CASTOR1 (NP_001032755.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MELH ILEH RVRV LSVA RPGL WLYT HPLI KLLF LPRR SRCK FFSL TETP EDYT LMVD EEGF KELP PSEF LQVA EATW LVLN VSSH SGAA VQAA GVTK IARS VIAP LAEH HVSV LMLS TYQT DFIL VREQ DLSV VIHT LAQE FDIY REVG GEPV PVTR DDSS NGFP RTQH GPSP TVHP IQSP QNRF CVLT LDPE TLPA IATT LIDV LFYS HSTP KEAA SSSP EPSS ITFF AFSL IEGY ISIV MDAE TQKK FPSD LLLT SSSG ELWR MVRI GGQP LGFD ECGI VAQI AGPL AAAD ISAY YIST FNFD HALV PEDG IGSV IEVL QRRQ EGLA S |
| Cellular Location: | |
| Calculated MW: | 36kDa |
| Observed MW: | 36KDa |
| Synonyms: | GATSL3 |
| Background: | Functions as an intracellular arginine sensor within the amino acid-sensing branch of the TORC1 signaling pathway. As a homodimer or a heterodimer with CASTOR2, binds and inhibits the GATOR subcomplex GATOR2 and thereby mTORC1. Binding of arginine to CASTOR1 allosterically disrupts the interaction of CASTOR1-containing dimers with GATOR2 which can in turn activate mTORC1 and the TORC1 signaling pathway. |
 | Immunofluorescence analysis of U2OS cells using CASTOR1 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of PC-12 cells using CASTOR1 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using CASTOR1 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunohistochemistry of paraffin-embedded rat ovary using CASTOR1 Rabbit pAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded rat brain using CASTOR1 Rabbit pAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Western blot analysis of extracts of Mouse thymus, using at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s. |
Related Products
Anti-RANBP3 Antibody (CAB0092)
OverviewProduct Name:RANBP3 Rabbit mAbProduct Code:CAB0092Size:20uL, 50uL, 100uLSynonyms:RANBP3, ran-binding protein 3
Anti-QK1 Antibody (CAB0193)
OverviewProduct Name:QK1 Rabbit mAbProduct Code:CAB0193Size:20uL, 50uL, 100uLSynonyms:QKI, Hqk, QK, QK1, QK3, hqkI, protein quaking
Anti-DEK Antibody (CAB0315)
OverviewProduct Name:DEK Rabbit mAbProduct Code:CAB0315Size:20uL, 50uL, 100uLSynonyms:D6S231EApplications:WB,
Anti-EXOSC7 Antibody (CAB0501)
OverviewProduct Name:EXOSC7 Rabbit mAbProduct Code:CAB0501Size:20uL, 50uL, 100uLSynonyms:EXOSC7, EAP1, RRP42, Rrp42p, hRrp42p, p8, exoso
Anti-Olig3 Antibody (CAB0600)
OverviewProduct Name:Olig3 Rabbit mAbProduct Code:CAB0600Size:20uL, 50uL, 100uLSynonyms:OLIG3, Bhlhb7, bHLHe20, oligodendrocyte transcri