Antibody Name:	Anti-CAST Antibody [KO Validated]
Antibody SKU:	CAB7634
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 550-650 of human CAST (NP_001177371.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, SW480, MCF-7, HepG2, Mouse lung, Rat liver

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 550-650 of human CAST (NP_001177371.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SDKD LDDA LDKL SDSL GQRQ PDPD ENKP MEDK VKEK AKAE HRDK LGER DDTI PPEY RHLL DDNG QDKP VKPP TKKS EDSK KPAD DQDP IDAL SGDL DSCP S
Gene ID:	831
Uniprot:	P20810
Cellular Location:
Calculated MW:	63kDa/71- 84kDa
Observed MW:	130kDa

Synonyms:

CAST, BS-17, PLACK

Background:

The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described.

UniProt Protein Function:	calpastatin: Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue. Belongs to the protease inhibitor I27 (calpastatin) family. 7 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Inhibitor Chromosomal Location of Human Ortholog: 5q15 Cellular Component: membrane; cytoplasm; cytosol Molecular Function:protein binding; endopeptidase inhibitor activity; cysteine protease inhibitor activity Disease: Peeling Skin With Leukonychia, Acral Punctate Keratoses, Cheilitis, And Knuckle Pads
NCBI Summary:	The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010]
UniProt Code:	P20810
NCBI GenInfo Identifier:	126302556
NCBI Gene ID:	831
NCBI Accession:	P20810.4
UniProt Related Accession:	P20810
Molecular Weight:	63kDa; 71-84kDa
NCBI Full Name:	Calpastatin
NCBI Synonym Full Names:	calpastatin
NCBI Official Symbol:	CAST
NCBI Official Synonym Symbols:	BS-17; PLACK
NCBI Protein Information:	calpastatin
UniProt Protein Name:	Calpastatin
UniProt Synonym Protein Names:	Calpain inhibitor; Sperm BS-17 component
Protein Family:	Calpastatin
UniProt Gene Name:	CAST
UniProt Entry Name:	ICAL_HUMAN

	Western blot analysis of extracts of various cell lines, using CAST antibody (CAB7634) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Western blot analysis of extracts from normal (control) and CAST knockout (KO) HeLa cells, using CAST antibody (CAB7634) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Antibodies	ELISA Kits
Anti-CAST Antibody (CAB0097)	Human Calpastatin (CAST) ELISA Kit
Anti-CAST Antibody (CAB13683)
Anti-CAST Antibody (CAB16789)
Anti-CAST Antibody (CAB16790)
Anti-CAST Antibody (CAB16791)