Antibody Name:	Anti-CASC5 Antibody
Antibody SKU:	CAB13108
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CASC5 (NP_733468.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Rat
Positive Samples:	HepG2, U-87MG, HeLa, Jurkat

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CASC5 (NP_733468.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDGV SSEA NEEN DNIE RPVR RRHS SILK PPRS PLQD LRGG NERV QESN ALRN KKNS RRVS FADT IKVF QTES HMKI VRKS EMEG CSAM VPSQ LQLL PPGF KRFS CLSL PETE TGEN LLLI QNKK LEDN YCEI TGMN TLLS APIH TQMQ QKEF SIIE HTRE RKHA NDQT VIFS DENQ MDLT SSHT VMIT KGLL DNPI SEKS TKID TTSF LANL KLHT EDSR MKKE VNFS VDQN TSSE NKID FNDF IKRL KTGK CSAF PDVP DKEN FEIP IYSK EPNS ASST HQMH VSLK EDEN NSNI TRLF
Gene ID:	57082
Uniprot:	Q8NG31
Cellular Location:	Chromosome, Nucleus, centromere, kinetochore
Calculated MW:	195kDa/205kDa/262kDa/265kDa
Observed MW:	300kDa

Synonyms:

AF15Q14, CASC5, CT29, D40, MCPH4, PPP1R55, Spc7, hKNL-1, hSpc105, KNL1

Background:

The protein encoded by this gene is a component of the multiprotein assembly that is required for creation of kinetochore-microtubule attachments and chromosome segregation. The encoded protein functions as a scaffold for proteins that influence the spindle assembly checkpoint during the eukaryotic cell cycle and it interacts with at least five different kinetochore proteins and two checkpoint kinases. In adults, this gene is predominantly expressed in normal testes, various cancer cell lines and primary tumors from other tissues and is ubiquitously expressed in fetal tissues. This gene was originally identified as a fusion partner with the mixed-lineage leukemia (MLL) gene in t(11;15)(q23;q14). Mutations in this gene cause autosomal recessive primary microcephaly-4 (MCPH4). Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described but their biological validity has not been confirmed.

UniProt Protein Function:	Performs two crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Required for attachment of the kinetochores to the spindle microtubules. Directly links BUB1 and BUB1B to kinetochores. Part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. Acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore.
NCBI Summary:	The protein encoded by this gene is a component of the multiprotein assembly that is required for creation of kinetochore-microtubule attachments and chromosome segregation. The encoded protein functions as a scaffold for proteins that influence the spindle assembly checkpoint during the eukaryotic cell cycle and it interacts with at least five different kinetochore proteins and two checkpoint kinases. In adults, this gene is predominantly expressed in normal testes, various cancer cell lines and primary tumors from other tissues and is ubiquitously expressed in fetal tissues. This gene was originally identified as a fusion partner with the mixed-lineage leukemia (MLL) gene in t(11;15)(q23;q14). Mutations in this gene cause autosomal recessive primary microcephaly-4 (MCPH4). Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described but their biological validity has not been confirmed. [provided by RefSeq, Jan 2013]
UniProt Code:	Q8NG31
NCBI GenInfo Identifier:	223590239
NCBI Gene ID:	57082
NCBI Accession:	Q8NG31.3
UniProt Secondary Accession:	Q8NG31,Q8NHE1, Q8WXA6, Q9HCK2, Q9NR92,
UniProt Related Accession:	Q8NG31
Molecular Weight:	205,826 Da
NCBI Full Name:	Protein CASC5
NCBI Synonym Full Names:	kinetochore scaffold 1
NCBI Official Symbol:	KNL1
NCBI Official Synonym Symbols:	D40; CT29; Spc7; CASC5; MCPH4; hKNL-1; AF15Q14; PPP1R55; hSpc105
NCBI Protein Information:	protein CASC5
UniProt Protein Name:	Protein CASC5
UniProt Synonym Protein Names:	ALL1-fused gene from chromosome 15q14 protein; AF15q14; Bub-linking kinetochore protein; Blinkin; Cancer susceptibility candidate gene 5 protein; Cancer/testis antigen 29; CT29; Kinetochore-null protein 1; Protein D40/AF15q14
Protein Family:	Protein
UniProt Gene Name:	CASC5
UniProt Entry Name:	CASC5_HUMAN

	Western blot analysis of extracts of various cell lines, using CASC5 antibody (CAB13108) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat testis using CASC5 antibody (CAB13108) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon using CASC5 antibody (CAB13108) at dilution of 1:100 (40x lens).