Cell Biology Antibodies 16
Anti-CAS9 Antibody (CAB14997)
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- SKU:
- CAB14997
- Product Type:
- Antibody
- Reactivity:
- Bacteria - Streptococcus
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-CAS9 Antibody |
| Antibody SKU: | CAB14997 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Streptococcus pyogenes |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of streptococcus pyogenes CAS9 |
| Application: | WB IF |
| Recommended Dilution: | WB 1:5000 - 1:10000 IF 1:50 - 1:200 |
| Reactivity: | Streptococcus pyogenes |
| Positive Samples: | HeLa |
| Immunogen: | Recombinant protein of streptococcus pyogenes CAS9 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 901176 |
| Uniprot: | |
| Cellular Location: | |
| Calculated MW: | |
| Observed MW: | 150kDa |
| Synonyms: | CAS9 |
| Background: |
 | Western blot analysis of extracts of wild HeLa cells and Hela transfected with cas9, using CAS9 antibody (CAB14997) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of HeLa cells transfected with Cas9 (upper left) and untreated HeLa cells (upper right) using CAS9 Rabbit pAb (red, A14997) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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