Antibody Name:	Anti-CAPNS1 Antibody
Antibody SKU:	CAB6539
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 69-268 of human CAPNS1 (NP_001740.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, U-251MG, SKOV3, Mouse lung, Mouse heart, Mouse spleen, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 69-268 of human CAPNS1 (NP_001740.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ISEA AAQY NPEP PPPR THYS NIEA NESE EVRQ FRRL FAQL AGDD MEVS ATEL MNIL NKVV TRHP DLKT DGFG IDTC RSMV AVMD SDTT GKLG FEEF KYLW NNIK RWQA IYKQ FDTD RSGT ICSS ELPG AFEA AGFH LNEH LYNM IIRR YSDE SGNM DFDN FISC LVRL DAMF RAFK SLDK DGTG QIQV NIQE WLQL TMYS
Gene ID:	826
Uniprot:	P04632
Cellular Location:	Cell membrane, Cytoplasm
Calculated MW:	28kDa
Observed MW:	28kDa

Synonyms:

CAPNS1, CALPAIN4, CANP, CANPS, CAPN4, CDPS, CSS1

Background:

This gene is a member of the calpain small subunit family. Calpains are calcium-dependent cysteine proteinases that are widely distributed in mammalian cells. Calpains operate as heterodimers, comprising a specific large catalytic subunit (calpain 1 subunit in Calpain I, and calpain 2 subunit in Calpain II), and a common small regulatory subunit encoded by this gene. This encoded protein is essential for the stability and function of both calpain heterodimers, whose proteolytic activities influence various cellular functions including apoptosis, proliferation, migration, adhesion, and autophagy. Calpains have been implicated in neurodegenerative processes, such as myotonic dystrophy. A pseudogene of this gene has been defined on chromosome 1. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	CAPNS1: Regulatory subunit of the calcium-regulated non- lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
UniProt Protein Details:	Protein type:Protease; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 19q13.12 Cellular Component: membrane; cytoplasm; plasma membrane; cytosol Molecular Function:protein binding; calcium-dependent cysteine-type endopeptidase activity; calcium ion binding Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; positive regulation of cell proliferation; proteolysis
NCBI Summary:	This gene is a member of the calpain small subunit family. Calpains are calcium-dependent cysteine proteinases that are widely distributed in mammalian cells. Calpains operate as heterodimers, comprising a specific large catalytic subunit (calpain 1 subunit in Calpain I, and calpain 2 subunit in Calpain II), and a common small regulatory subunit encoded by this gene. This encoded protein is essential for the stability and function of both calpain heterodimers, whose proteolytic activities influence various cellular functions including apoptosis, proliferation, migration, adhesion, and autophagy. Calpains have been implicated in neurodegenerative processes, such as myotonic dystrophy. A pseudogene of this gene has been defined on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
UniProt Code:	P04632
NCBI GenInfo Identifier:	115612
NCBI Gene ID:	826
NCBI Accession:	P04632.1
UniProt Related Accession:	P04632
Molecular Weight:	28kDa
NCBI Full Name:	Calpain small subunit 1
NCBI Synonym Full Names:	calpain small subunit 1
NCBI Official Symbol:	CAPNS1
NCBI Official Synonym Symbols:	CANP; CDPS; CSS1; CANPS; CAPN4; CALPAIN4
NCBI Protein Information:	calpain small subunit 1
UniProt Protein Name:	Calpain small subunit 1
UniProt Synonym Protein Names:	Calcium-activated neutral proteinase small subunit; CANP small subunit; Calcium-dependent protease small subunit; CDPS; Calcium-dependent protease small subunit 1; Calpain regulatory subunit
Protein Family:	Calpain
UniProt Gene Name:	CAPNS1
UniProt Entry Name:	CPNS1_HUMAN

	Western blot analysis of extracts of various cell lines, using CAPNS1 Antibody (CAB6539) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human liver cancer using CAPNS1 antibody (CAB6539) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon using CAPNS1 antibody (CAB6539) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal cancer using CAPNS1 antibody (CAB6539) at dilution of 1:100 (40x lens).