Cell Biology Antibodies 11
Anti-CAPN5 Antibody (CAB7428)
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- SKU:
- CAB7428
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-CAPN5 Antibody |
| Antibody SKU: | CAB7428 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 361-640 of human CAPN5 (NP_004046.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SKOV3, Mouse thymus, Mouse brain, Mouse spinal cord |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 361-640 of human CAPN5 (NP_004046.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GAWT LHED PRQN RGGG CINH KDTF FQNP QYIF EVKK PEDE VLIC IQQR PKRS TRRE GKGE NLAI GFDI YKVE ENRQ YRMH SLQH KAAS SIYI NSRS VFLR TDQP EGRY VIIP TTFE PGHT GEFL LRVF TDVP SNCR ELRL DEPP HTCW SSLC GYPQ LVTQ VHVL GAAG LKDS PTGA NSYV IIKC EGDK VRSA VQKG TSTP EYNV KGIF YRKK LSQP ITVQ VWNH RVLK DEFL GQVH LKAD PDNL QALH TLHL RDRN SRQP SNLP GTVA VHIL SSTS LMAV |
| Gene ID: | 726 |
| Uniprot: | O15484 |
| Cellular Location: | |
| Calculated MW: | 73kDa |
| Observed MW: | 73kDa |
| Synonyms: | CAPN5, ADNIV, HTRA3, VRNI, nCL-3, calpain-5 |
| Background: | Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3. |
| UniProt Protein Function: | CAPN5: Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3. [provided by RefSeq, Jul 2008] |
| UniProt Protein Details: | Protein type:Protease; EC 3.4.22.- Chromosomal Location of Human Ortholog: 11q14 Cellular Component: cell surface; focal adhesion; cytoplasm Molecular Function:calcium-dependent cysteine-type endopeptidase activity Biological Process: proteolysis; signal transduction Disease: Vitreoretinopathy, Neovascular Inflammatory |
| NCBI Summary: | Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O15484 |
| NCBI GenInfo Identifier: | 28376972 |
| NCBI Gene ID: | 726 |
| NCBI Accession: | O15484.2 |
| UniProt Secondary Accession: | O15484,O00263, |
| UniProt Related Accession: | O15484 |
| Molecular Weight: | 640 |
| NCBI Full Name: | Calpain-5 |
| NCBI Synonym Full Names: | calpain 5 |
| NCBI Official Symbol: | CAPN5 |
| NCBI Official Synonym Symbols: | VRNI; ADNIV; HTRA3; nCL-3 |
| NCBI Protein Information: | calpain-5; new calpain 3; calpain htra-3 |
| UniProt Protein Name: | Calpain-5 |
| UniProt Synonym Protein Names: | Calpain htra-3; New calpain 3; nCL-3 |
| Protein Family: | Calpain |
| UniProt Gene Name: | CAPN5 |
| UniProt Entry Name: | CAN5_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CAPN5 antibody (CAB7428) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s. |
 | Immunofluorescence analysis of NIH-3T3 cells using CAPN5 antibody (CAB7428) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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